Genotyping and primer details


Genotyping and primer details for SALK_124063c - At4g14455
Line ID SALK_124063c
Locus At4g14455 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:8310804 of line SALK_124063c Chr4:8310804
   
Insertion allele amplicon LBb3 - 25rb
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 25rb: 5'-AAAACTTACGCTTCTGGAATTGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:8310371
   
wt amplicon (over InsPos) 33rz - 25rb
wt amplicon start Chr4:8310371
wt amplicon end Chr4:8311394
wt amplicon size 1024 bp
Locus specific primer 25rb: 5'-AAAACTTACGCTTCTGGAATTGAA-3'
2nd locus specific primer 33rz: 5'-ACTTACATCTCAGGCAAGCGTTAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11