Genotyping and primer details


Genotyping and primer details for SALK_126353c - At5g64400
Line ID SALK_126353c
Locus At5g64400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:25749033 of line SALK_126353c Chr5:25749033
   
Insertion allele amplicon LBb3 - 16ke
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 16ke: 5'-TTACATCTTGGAAAGCCTTAGCAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:25749798
   
wt amplicon (over InsPos) 13ls - 16ke
wt amplicon start Chr5:25748815
wt amplicon end Chr5:25749798
wt amplicon size 984 bp
Locus specific primer 16ke: 5'-TTACATCTTGGAAAGCCTTAGCAT-3'
2nd locus specific primer 13ls: 5'-TATAAATAACCGCCTCCACAAAAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11