Sequences from confirmation amplicon


Confirmation sequences for line SALK_126353c and hit At5g64400 are shown.
Line ID SALK_126353c
Locus At5g64400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25749033
Primers used for PCR 16ke - LBb3, show primer details
   
Sequence Name 08-K113843-SALK_126353C-16ke
GenBank Accession LN484495 [GenBank]
Sequencing primer gene specific primer, 16ke: 5'-TTACATCTTGGAAAGCCTTAGCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K113843-SALK_126353C-16ke
GGGCTGCAGATGCAGAAGCAGCCTCAACAGCCTCATGCTGAATGGTTCGTGGACCCATCA
CAGAATCAACAGCCCTGTGTGCAACTGCACTTCCAGTACCAAAAGCCATACCTATTTCCC
AAAAACACAAACAAACAGGTAAGGAAAGACGATTCCAGACAAAACAATTGGGGTCGTATA
TAGAGAGACAAATAATAGCTTCAAAATTAAACAGAAATAATTAGGCTGATTTACCCTGAG
CAATGGTTGAACCAATGCCTGAGAACATGCCACCACCACCACCGCTGGCCTGAGCTGGAG
CTGGAGGAGGTGCACGGTGTACTGCAAAACAAAACCCAGAAAGAATGATTTATAAATAAT
TGAATCCAGCGGAATGGATTGAATAAGTCATAACTAAGAGCAAAAAAACACAATTTCGGA
GACAAAGATTTGTACCAGGTTGAGGAGCTGGGCTGCGTGCAGCAGCAGGACGAGGGCGAG
GGGCAGATCTTCCTGCACAAATGAAGTCAAAATGAGAGATATATCACAAAAACAAGGAAT
TTTTTTTTTTGACATCAACAATGAAATCAATGACAACCATGTCTCGAATTGAAAACTAAA
CCAAGCCTAATACCTTAAAAATATTTGATTATCTAATATCCAATAAGGTCAATAACAAGG
TAAACCCTAGATCGGTGCGATACACGCATAATCGAAACAAACCCAAACCAAAACAAAATT
GACGCTAAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACGGGGGTGGTTTTTC
TTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCGGGCCCTAAGAGAGTT
GCAGCAAGCGGTCCACGCTGGTTTGCCCCACCAGGCAAAAATCCTGTTTGA
   
Sequence Name 04-K113842-SALK_126353C-R210
GenBank Accession LN484372 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K113842-SALK_126353C-R210
GGTTCGTTTCGATTATGCGTGTATCGCACCGATCTACGGTTTACCTTGTTATTGACCTTA
TCGGATATTAGATAATCAAATATTCTTAAGGTATTAGGCTTGGTTTAGTTTCCAATTCGA
GACATGGTTGTCATTGATTTCATTGTTGATGTCAAAAAAAAAAATTCCTTGTTTTTGTGA
TATATCTCTCATTTTGACTTCATTTGTGCAGGAAAATCTGCCCCTCGCCCTCGTCCTGCT
GCTGCACGCACCCCACCTCCTCAACCGGGTACAAATCTTTGTCTCCAAAATTGTGTTTTT
TTGCTCTTAGTTATAACTTATTCAATCCATTCCGCTGGATTCAATTATTTATAAATCATT
CTTTCGGGGTTTTGTTTTGCAGTACACCGTGCACCTCCTCCAGCTCCAGCTCAGGCCACC
GGTGGTGGTGGTGGCATGTTCTCAGGCATTGGTTCAACCATTGCTCAGGGTAAATCACCC
AAATTATTTCTGTTTAATTTTAAACCTATAATTTGTCTCTCTATATACAACCCCAATTGT
TTTGTCGGGAATCGTCTTCCCTAACCGGTTTGTTTGTGTTTTTGGAAAATAGGTATGGCT
TTTGGTACTGAAATTGCATTTGCACACAGGGCTGTTAATTCTGTGATGGGTCCACAAACC
ATTCACCATGAGGCTGTTGAGGCTGCTTCTGCATCTGCACCCCCTGCTGAAACCGCCATG
CTTTCTAGCACCTGTAACATTCATGCTAAG

Last Updated on 06.12.2018 16:11