Genotyping and primer details


Genotyping and primer details for SALK_147277c - At5g51600
Line ID SALK_147277c
Locus At5g51600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:20961475 of line SALK_147277c Chr5:20961475
   
Insertion allele amplicon LBb3 - 07ko
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 07ko: 5'-ATTATTTCATGGCTGACCATTTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:20962048
   
wt amplicon (over InsPos) 01ll - 07ko
wt amplicon start Chr5:20960984
wt amplicon end Chr5:20962048
wt amplicon size 1065 bp
Locus specific primer 07ko: 5'-ATTATTTCATGGCTGACCATTTTT-3'
2nd locus specific primer 01ll: 5'-ACCTTTTACAAACCAAACCACATT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11