Sequences from confirmation amplicon


Confirmation sequences for line SALK_147277c and hit At5g51600 are shown.
Line ID SALK_147277c
Locus At5g51600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:20961475
Primers used for PCR 07ko - LBb3, show primer details
   
Sequence Name 49-K113814-SALK_147277C-R210
GenBank Accession LN485481 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K113814-SALK_147277C-R210
CGTCATTTGTTTCCCACAATGTGTTATAATGGGTGATTGTTTCCTTGAGGGTACTCTTGC
AATGTCAAGCCCTCTTCTTCCTGCAGGAATAAGATTCATTTGCCAATGCAATTAGGAAAT
CTATTCATACTATGATGATAACCAATGATTTACATTTGATTAAGAGACTTAGTTACCATT
TGATAAAGCTCCATCATTATGTCGGTGATCTGCTTTCTTGTTAGGCTTAGGAGTCTGATG
CATTGCTGCTCCTAAGGAAAGTCTTCTGTTAGATGCACCACCGGTCGACATTCTTGGAGC
CTTTTTACCACCAAGGGGTTTAGATGGGCTGGGTTTGGACCCGTAAAGTGCCTCTTGTTC
TGCTATGAGCTGTCCTTGAAGCTTTTTCTGATCCTTTAATATGTAGAAAAAAGTTGCATC
ACATAGAGTTGGATTAGGAAGATGATATTGGGTCAAAAGTTTCTGGAAACAATAAATAGA
GGACTTATACGTACCCGTTGCCGACGGTGTTCTTCTTCTCTTTCCTGCCTGAGTATGTTA
TACTCCTCAAGCATAGAAAGGAGACGGATCTAGACACAGAAAAAAAAATGGTCAGCAATG
AAATA
   
Sequence Name 62-K113815-SALK_147277C-07ko
GenBank Accession LN485794 [GenBank]
Sequencing primer gene specific primer, 07ko: 5'-ATTATTTCATGGCTGACCATTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K113815-SALK_147277C-07ko
TATACATACTCAGGCAGGAAAGAGAAGAAGACACCGCCCTGTAACGGGTACGTATAAGTC
CTCTATTTATTGTTTCCAGAAACTTTTGACCCAATATCATCTTCCTAATCCAACTCTATG
TGATGCAACTTTTTTCTACATATTAAAGGATCAGAAAAAGCTTCAAGGACAGCTCATAGC
AGAACAAGAGGCACTTTACGGGTCCAAACCCAGCCCATCTAAACCCCTTGGTGGTAAAAA
GGCTCCAAGAATGTCGACCGGTGGTGCATCTAACAGAAGACTTTCCTTAGGAGCAGCAAT
GCATCAGACTCCTAAGCCTAACAAGAAAGCAGATCACCGACATAATGATGGAGCTTTATC
AAATGGTAACTAAGTCTCTTAATCAAATGTAAATCATTGGTTATCATCATAGTATGAATA
GATTTCCTAATTGCATTGGCAAATGAATCTTATTCCTGCAGGAAGAAGAGGGCTTGACAT
TGCAGGACTACCCTCAAGGAAACAATCACCCATTAATAACACATTGTGGTGTAAACAAAT
TGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTT
CTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGT
TGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTT
CCGAAATCGGCAAAA

Last Updated on 06.12.2018 16:11