Genotyping and primer details


Genotyping and primer details for SALK_150463c - At3g54960
Line ID SALK_150463c
Locus At3g54960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:20366040 of line SALK_150463c Chr3:20366040
   
Insertion allele amplicon LBb3 - 35dh
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 35dh: 5'-AAGCTTGATGAGAGAGGCATAGAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:20365229
   
wt amplicon (over InsPos) 17lt - 35dh
wt amplicon start Chr3:20365229
wt amplicon end Chr3:20366342
wt amplicon size 1114 bp
Locus specific primer 35dh: 5'-AAGCTTGATGAGAGAGGCATAGAG-3'
2nd locus specific primer 17lt: 5'-GATTTGGCTCAGAAGTACGAGATT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11