Sequences from confirmation amplicon


Confirmation sequences for line SALK_013055c and hit At1g05900 are shown.
Line ID SALK_013055c
Locus At1g05900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1788285
Primers used for PCR 23mh - LBb3, show primer details
   
Sequence Name 77-K114279-SALK_013055C-23mh
GenBank Accession LN486176 [GenBank]
Sequencing primer gene specific primer, 23mh: 5'-GATCAAAACAGCAGGTTTTCTTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K114279-SALK_013055C-23mh
CATTTTGGGTACATTTATTCTTTACGGAAAACGCGATGTCCCTAAGATGTTTATTTAGGG
CTAGGCATACCACCTGAAAACTGGGAAAAGGTGCTCGAGGGTATTCGAAAAATGAAACCT
TCAGAAGAAGCACCAGTTAATGCTGTAGAATGTGATAGAACCGGAAGCTTTTTACCTCCA
AAGGTAGAAATATCTTACACTAAATGGAATTGAGGTATTGCTGAGTTTGTGCCTATTGGT
TTTATGTGGAAGTATACAATGAGATAGTTTCATTGCTTTCTCAAATATTACTTCCCATTT
GCTTTTCATTATTACATGGTAGTACTCTATGCATATATTGGGAACCAAGTTCCAAGTGGT
TTACCGTGAATTAGTAACAAATGCTAGCTTATGATTAGAAAATTTTCATATTCTGAATTC
TTGCTTCCTTGAAAGGTCGATGGAAGGTTTCCCTTCTTGTTTTTCCTTTTGATCTTATCT
CTCCCTATTTCTAGGAAAGGCGATTTTATGTGCTCATAGGAACACTTCTTTCAAGTCAGA
CTAAAGAGCACATAACTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGC
GGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGA
TTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCC
AGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 63-K114278-SALK_013055C-R210
GenBank Accession LN485826 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K114278-SALK_013055C-R210
CAGTTATGTGCTCTTTAGTCTGACTTGAAAGAAGTGTTCCTATGAGCACATAAAATCGCC
TTTCCTAGAAATAGGGAGAGATAAGATCAAAAGGAAAAACAAGAAGGGAAACCTTCCATC
GACCTTTCAAGGAAGCAAGAATTCAGAATATGAAAATTTTCTAATCATAAGCTAGCATTT
GTTACTAATTCACGGTAAACCACTTGGAACTTGGTTCCCAATATATGCATAGAGTACTAC
CATGTAATAATGAAAAGCAAATGGGAAGTAATATTTGAGAAAGCAATGAAACTATCTCAT
TGTATACTTCCACATAAAACCAATAGGCACAAACTCAGCAATACCTCAATTCCATTTAGT
GTAAGATATTTCTACCTTTGGAGGTAAAAAGCTTCCGGTTCTATCACATTCTACAGCATT
AACTGGTGCTTCTTCTGAAGGTTTCATTTTTCGAATACCCTCGAGCACCTTTTCCCAGTT
TTCAGGTGGTATGCCTAGCCCTAAATAAACATCTTAGGGACATCGCGTTTTCCGTAAAGA
ATAAATGTACCCAAAATGCCACTAAGAGTAGTAGAGTAGAGTCTCGTGAAAGAAAACC

Last Updated on 06.12.2018 16:11