Sequences from confirmation amplicon


Confirmation sequences for line SALK_122484c and hit At1g48450 are shown.
Line ID SALK_122484c
Locus At1g48450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:17910382
Primers used for PCR 48fx - LBb3, show primer details
   
Sequence Name 07-K112347-SALK_122484C-R210
GenBank Accession LN484460 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K112347-SALK_122484C-R210
CATTTGTTGGCAAAGTTCCGACTTTTTGCTTCTCCAGAGGCTAATCATGGACGCTGTGGC
GGCGAATTTGTTTCAATCTCCTTCCTTACTGCGTCAAACTTCCGAAAGGTTGATATTTTC
GACGCCCAACTGTCCTGGCTTCATGCGAATTAGCTCCGGACCTCAATTTCGTCTCAGAGT
AGGTCTTATTTCTACTGAGTTTGATTTACTAAACCCTAGACAACATTGACATTTATAGCT
TTCCCGAATCATCTTTGTTTGTGCTTAATCTACCCCATAATCGTATATCTTCCAATGAAA
TTCGAGAATTCAGATTCTGTGTAAAAAAATGGAACTTTTAAGGTTTCTTCGTGTAAATTT
CTCATAAGTAAAAAAATTGGCTTGACTATATTGTAATTTGAATCTGTTGTCTCTCTGTAT
GCAGCAAAACTCTTCTCTTAAATTCTCGAGACCATTTCAAAGCGGAACAACTTGTGTTAA
ATCTCGGAGAAGCTTTGTGGTTAAAGCTTCAGCTTCTGGTGATGCTTCAACAGAATCAAT
CGCGCCGCTTCAGTTGAAGTCTCCTGTGGGACAGTTTCTGTCACAGATATTAGTGAGCCA
TCCTCATCTTGTTCCAGCAGCTGTTGAACAGCAGCTTGAACAGCTCCAAATTGATCGAGA
CGCTGAAGAACAGAGTAAAGATGCATCCTCTGTCCTTGGAACAGACATTGTTCTCTACAG
GTTTGTCATATGCTGATATTAATTACCACTCGGCTATTATATAGGTTTGGATATTGAATT
AGATTGTTTCTTGGG
   
Sequence Name 16-K112348-SALK_122484C-48fx
GenBank Accession LN484720 [GenBank]
Sequencing primer gene specific primer, 48fx: 5'-TTCTCCTAAAGACCCAAGAAACAA-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K112348-SALK_122484C-48fx
AACTATTATAGCCGAGTGGTATTAATATCAGCATATGACAAACCTGTAGAGAACAATGTC
TGTTCCAAGGACAGAGGATGCATCTTTACTCTGTTCTTCAGCGTCTCGATCAATTTGGAG
CTGTTCAAGCTGCTGTTCAACAGCTGCTGGAACAAGATGAGGATGGCTCACTAATATCTG
TGACAGAAACTGTCCCACAGGAGACTTCAACTGAAGCGGCGCGATTGATTCTGTTGAAGC
ATCACCAGAAGCTGAAGCTTTAACCACAAAGCTTCTCCGAGATTTAACACAAGTTGTTCC
GCTTTGAAATGGTCTCGAGAATTTAAGAGAAGAGTTTTGCTGCATACAGAGAGACAACAG
ATTCAAATTACAATATAGTCAAGCCAATTTTTTTACTTATGAGAAATTTACACGAAGAAA
CCTTAAAAGTTCCATTTTTTTACACAGAATCTGAATTCTCGAATTTCATTGGAAGATATA
CGATTATGGGGTAGATTAAGCACAAACAAAGATGATTCGGGAAAGCTATAAATGTCAATG
TTGTCTAGGGTTTAGTAAATCAAACTCAGTAGAAATAAGACCTACTCTGAGACGAAATTG
AGGTCCGGAGCTAATTCGCATGAAGCCAGGACAGTTGGGCGTCGAAAATATCAACCTTTC
GGAAGTTTGACGCAGTAAGGAAGGAGATTGAAACAAATTCGCCGCCACAGCGTCCATGAT
TAGCCTCTGGGAGAAGCAAAAAGTCGGAACTTTGGCCAACAAATTGACGCTNAGACAACT
TAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGA
CGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCA
CGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCC

Last Updated on 06.12.2018 16:11