Sequences from confirmation amplicon


Confirmation sequences for line 293B08 and hit At2g28800 are shown.
Line ID 293B08
Locus At2g28800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:12357197
Primers used for PCR PH53 - 8474, show primer details
   
Sequence Name 03-K017512-022-293-B08C-PH53
GenBank Accession LN487272 [GenBank]
Sequencing primer gene specific primer, PH53: 5'-TGATGATCCTGCACAGAAGAATAC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K017512-022-293-B08C-PH53
AGTTTCTTCCACTCATGATCGGTTACTTTGCATTGTCTGTCCCATCAGGACTATCTATTT
ACTGGTTGGTTTCCCTCTCCTTGAAACTTCTCATCTAACTGTTTTATCCATGCCCTTGAT
CTACTCATTAAATGGAGTATAAGAAAGATTTTCTCTAGTAATTAACCTAGATGGCTTCAT
TCTCAAAATGTACGTAAGACGGGTTAGAATTTCGAGGAATACGAAGCTTGTTTGATAAGG
AATCATCTAGTGTGACTACATTTTGTAACATCAATATTATTGTATATGACTGTGTTTATT
GGTAGGATCTGAGTTTTATTGAACATTGCTGGTCCAGGATATATTCAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATA
AAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAG
CAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC
   
Sequence Name 07-K017512-022-293-B08C-8409
GenBank Accession LN489042 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K017512-022-293-B08C-8409
AGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGGACCAGCAATGTTCAAT
AAAACTCAGATCCTACCAATAAACACAGTCATATACAATAATATTGATGTTACAAAATGT
AGTCACACTAGATGATTCCTTATCAAACAAGCTTCGTATTCCTCGAAATTCTAACCCGTC
TTACGTACATTTTGAGAATGAAGCCATCTAGGTTAATTACTAGAGAAAATCTTTCTTATA
CTCCATTTAATGAGTAGATCAAGGGCATGGATAAAACAGTTAGATGAGAAGTTTCAAGGA
GAGGGAAACCAACCAGTAAATAGATAGTCCTGATGGGACAGACAATGCAAAGTAACCGAT
CATGAGTGGAAGAAACTTGAAAACAAGAAGCGTATTCTTCTGTGCAGGATCATC

Last Updated on 06.12.2018 16:11