Sequences from confirmation amplicon


Confirmation sequences for line 132C08 and hit MCK7 are shown.
Line ID 132C08
Locus MCK7 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23584471
Primers used for PCR 02md - 8474, show primer details
   
Sequence Name 66-K114263-0022-132-C08C-02md
GenBank Accession LN511666 [GenBank]
Sequencing primer gene specific primer, 02md: 5'-TAGTCAATTTTCCCTCAAGATGCT-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K114263-0022-132-C08C-02md
ATGAGAAAGGTTCGGATAGTCAGGAAACATGGTCTAGCAGAGTTCGGCCTCGTCGACCAA
CCCCTGTAACTCTGAGTGTATCTCCATTAAAGAAAGGCGGATTGGCGAAACCACATGTCA
GGAGACCAAAGAAATTCTGGAAGCCAGAAGAAGTGGAAGCTCTGAGGGAAGGAGTAAAAG
AGTATGCAAACACTCTTTGCCTAGTCCCGTACTCTATTATCAATATTCAATAGTGATACA
TCTTGGACCTCATTAAGATTATACCACTGGCTCATTTTGTTGTTGTTGTTGTGCAGGTAT
GGTAAATCATGGAAAGATATAAAGAATGGAAACCCTACAGTATTTGCGGAGAGGACAGAG
GTGAACATTTCCTTATTAACATTCATACTCACCATTTACTTGAACAAGATATATGCCAAG
TGTGAGTGATGTAAATGTTGTAACTTGGTAGGTGGATTTGAAAGATAAATGGAGGAACTT
AGTCGGTGGGTAGAGGTAACATCTTTTGTGAATCTTGTGCTATATGTTTTAAAATGCTGT
AGAGTTATTTACCTTTGTGTATTGTGATATATCAACTGTGGAGAACATGGTGGAGAAAGG
GCCATTCCCATGTTGTGTGGTGAATGATAATGATGAATGAGCAGAATATAATTATATGCT
TGTGGGAAAAATTCTGGCTGCAACCTCTGTAGTCAGTCAGTGTCAAACTTATACNGCTTT
TCTACATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTTATTACCAATTTTTTTAAAAAAAAAAAATGT
   
Sequence Name 23-K114262-0022-132-C08C-8409
GenBank Accession LN496650 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K114262-0022-132-C08C-8409
AAGCCGTATAAGTTTGACACTGACTGACTACAGAGGTTGCAGCCAGAATTTTTCCCACAA
GCATATAATTATATTCTGCTCATTCATCATTATCATTCACCACACAACNATGGGAATGGC
CNCTTTCTCCACCATGTTCTCCACAGTTGATATATCACAATACACAAAGGTAAATAACTC
TACAGCATTTTAAAACATATAGCACAAGATTCACAAAAGATGTTACCTCTACCCACCGAC
TAAGTTCCTCCATTTATCTTTCAAATCCACCTACCAAGTTACAACATTTACATCACTCAC
ACTTGGCATATATCTTGTTCAAGTAAATGGTGAGTATGAATGTTAATAAGGAAATGTTCA
CCTCTGTCCTCTCCGCAAATACTGTAGGGTTTCCATTCTTTATATCTTTCCATGATTTAC
CATACCTGCACAACAACAACAACAAAATGAGCCAGTGGTATAATCTTAATGAGGTCCAAG
ATGTATCACTATTGAATATTGATAATAGAGTACGGGACTAGGCAAAGAGTGTTTGCATAC
TCTTTTACTCCTTCCCTCAGAGCTTCCACTTCTTCTGGCTTCCAGAATTTCTTTGGTCTC
CTGACATGTGGTTTCGCCAATCCGCCTTTCTNTAATGGAGATACACTCAGAGTTACAGGG
GTTGGTCGACGAGGCCGAACTCTGCTAGACCATGTTTCCTGACTATCCGAACCTTTCTCA
TTTACATTATCCTGTTTTCACAATTAAACCACANATTCATGAACCAAAGAACAAGATTTT
TAGAAAGCATCTAGAGGGAAAATTGA

Last Updated on 06.12.2018 16:11