Sequences from confirmation amplicon


Confirmation sequences for line 004D08 and hit At3g24463 are shown.
Line ID 004D08
Locus At3g24463 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:8887800
Primers used for PCR 21do - 8474, show primer details
   
Sequence Name 29-K115331-0022-004-D08C-21do
GenBank Accession LN499419 [GenBank]
Sequencing primer gene specific primer, 21do: 5'-TCCAGCTCTTATGGGACTCTATGT-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K115331-0022-004-D08C-21do
GTTTCCTTTCATCTTGATCTCTGTTTTTTTTTTCATGTCTTAAAAAGTTCAAAACCGATA
ATCCGGTCCGGTTAGATCTTAAAAATCTAAATTATTTGACCCGGTTAAATCAAATAAAAA
CAGAATTAACCTACAAACTGATAGTTTGGTTGACCGAGCCGGTTAGTTGTTCAAATTAAA
AAATTTCACTTGGTTAAAAAGTTTGAAAATCAAATAAATAAAACATTGTTTCATTTATTT
CTCTTTTTTCCAAAGTTTTGACTTGGGGATTTGATTATTTTTTTGAAGTTATTCTCTACA
AAAATTTTTAAATTTATTTTGGGGTTTAGGTAACTTCTCTAAAGTTAGTATGGTTTAGGA
ATTAGGAGTTAGTAGTAAAACTAGTCAAGTAAAAAATATAGTTGGTTTTGGGAGTCTTTA
GCCACTATATATATGGTGGAGAAAATAGGCCAAAAACCATTCATTATCTTCCACTTCTGT
TTTCAAAGCTTCCATCGTCAAATCTCTCTGTCCCTGGAAATATGTCAAAAAACAACACTA
CTGCAAAAATTGTTAGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAAATGTCCGCA
   
Sequence Name 05-K115330-0022-004-D08C-8409
GenBank Accession LN488475 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K115330-0022-004-D08C-8409
TTTCATTGAATATATCCTGCTACAATCTCTGCAGTAGTGTTGTTCTCTGACATATCTCCA
GGGACAGAGAGATTTGACGATGGAAGCTTTGAAAACAGAAGTGGAAGATAATGAATGGTT
CTTGGCCTATCTTCTCCACCATATATATAGTGGCTAAAGACTCCCAAAACCAACTATATT
TTCTACTTGACTAGTTCTACTACTAACTCCTAATTCCTAAACCATACTAACTTTAGAGAA
GTTACCTAAACCACAAAATAAATTTAAAAATCTCTGTAGAGAATAACTTCAAAAAAATAA
TCAAATCCCCAAGTCAAAACTTAGGAAAAAAGAGAAATAAATGAAACAATGTTTTATTTA
TTTGATTTTCAAACTTTTTAACCAAGTGAAATTTTTTAATTTGAACAACTAACCGGCTCG
GTCAACCAAACTATCAGTTTGTAGGTTAATTCTGTTTTTATCTGATTTAACCGGGTCAAA
TAATTTAGATTTTTAAGATCTAACCGGACCGGATTATCGGTTTTGAACTTTTTAAGACAT
TGAAAAAAAAAACAGAGATCAAGATTGAAAGGAAACATAAACTTACCTTCGAATGGCACA
CCAGCATATAAACACAACATAGAGTCCCTTA

Last Updated on 06.12.2018 16:11