Sequences from confirmation amplicon


Confirmation sequences for line 005H11 and hit At5g26860 are shown.
Line ID 005H11
Locus At5g26860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:9454852
Primers used for PCR AF05 - 8474, show primer details
   
Sequence Name 69-K105142-0022-005-H11C-AF05
GenBank Accession LN512203 [GenBank]
Sequencing primer gene specific primer, AF05: 5'-AAATTTGAGTAAATGTGTCCCCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K105142-0022-005-H11C-AF05
CAAACTAGTATTGGGCGGTCTATTGCACGTGCTCTTAATCGCAAATTTTTCCGGTTCTCT
GTTGGAGGGTTAGCAGATGTCGCTGAGATCAAGGTATAATACCTTTGTTTTGGTATACTT
GCAAATATTAGTCAAACATTTGTGACTGAATTCTTTCCTAGAAACAAAAAACCATTCTTG
TAATTACATGTTTAGGGGCATCGTCGGACATATGTTGGCGCCATGCCAGGAAAGATGGTG
CAATGTCTAAAGAGTGTGGGGACAGCAAATCCTCTTGTTCTAATCGATGAGATTGATATT
TGATATTTGTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCC
   
Sequence Name 66-K105143-0022-005-H11C-8409
GenBank Accession LN511609 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K105143-0022-005-H11C-8409
TCGGACTGAGCATTTACATTGAATAATCCTGACAATATCAAATATCAATCTCATCGATTA
GAACAAGAGGATTTGCTGTCCCCACACTCTTTAGACATTGCACCATCTTTCCTGGCATGG
CGCCAACATATGTCCGACGATGCCCCTAAACATGTAATTACAAGAATGGTTTTTTGTTTC
TAGGAAAGAATTCAGTCACAAATGTTTGACTAATATTTGCAAGTATACCAAAACAAAGGT
ATTATACCTTGATCTCAGCGACATCTGCTAACCCTCCAACAGAGAACCGGAAAAATTTGC
GATTAAGAGCACGTGCAATAGACCGCCCAATACTAGTTTTGCCTACTCCAGGAGGGCCGG
AGAGGCAGATGATTTTCCCTGGGGACACATTANCTCAAATT

Last Updated on 06.12.2018 16:11