Genotyping and primer details


Genotyping and primer details for 005H11 - At5g26860
Line ID 005H11
Locus At5g26860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:9454852 of line 005H11 Chr5:9454852
   
Insertion allele amplicon 8474 - AF05
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AF05: 5'-AAATTTGAGTAAATGTGTCCCCAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:9454493
   
wt amplicon (over InsPos) AF06 - AF05
wt amplicon start Chr5:9454493
wt amplicon end Chr5:9455315
wt amplicon size 823 bp
Locus specific primer AF05: 5'-AAATTTGAGTAAATGTGTCCCCAG-3'
2nd locus specific primer AF06: 5'-GAAATCACCTCCATTCTGTCTAGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11