Sequences from confirmation amplicon


Confirmation sequences for line 011E09 and hit At1g44110 are shown.
Line ID 011E09
Locus At1g44110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:16775631
Primers used for PCR AS02 - 3144, show primer details
   
Sequence Name 05-K018333-022-011-E09C-AS02
GenBank Accession LN488152 [GenBank]
Sequencing primer gene specific primer, AS02: 5'-GGAAGCAGCTACTAGTGAAGGAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K018333-022-011-E09C-AS02
CATAGTGTACTCTAGTAGCGATAATCCCGCAATGTAGTTAGCCATACATTCTAGCTGCAT
CAATGGAGCTTCATGAACACCATGAGCAGCTCGAACAAAGCGTCTGAAAATTCAAGTTGT
ACGATCCAACTTCGACAAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGAT
CCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTG
CGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAATCCAC
TTGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCCACGATGCTCCTCGTGGGTGGGGG
TCCATCTTTG
   
Sequence Name 06-K018333-022-011-E09C-3144
GenBank Accession LN488598 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K018333-022-011-E09C-3144
CGTAGGGATGACGCACAATCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGT
TCATTTCATTTGGAGAGGACAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGT
TTAAACTATCAGTGTTTGTCGAAGTTGGATCGTACAACTTGAATTTTCAGACGCTTTGTT
CGAGCTGCTCATGGTGTTCATGAAGCTCCATTGATGCAGCTAGAATGTATGGCTAACTAC
ATTGCGGAATTATCGCTACTAGAGTACACTATGCTCTCTCATTCTCCTTCACTAGTAGCT
GCTTC
Primers used for PCR 52xh - 3144, show primer details
   
Sequence Name 54-K119070-0022-011-E09C-52xh
GenBank Accession LN508644 [GenBank]
Sequencing primer gene specific primer, 52xh: 5'-TTGATGTCTTACGGAAATCAAGAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K119070-0022-011-E09C-52xh
TAATTTATCAAATTCATTTATCACTTGATCAATAATAGCCATGAAATCTGCATAACACGT
TTGTTTGCATATTTAAGCAGATTGATAATAACTTTTCCCACTTTTACCAATCAAATCATA
TCACCTAATTACGTCACTTTCTAGAGGAAGCCAAAATAAATCAGCTTAACAAGTTTTCAA
AGTGAGATCGTAGATAGCTAATTTTATAAATAGACCAACAACAGCAAAATCATGTACAAT
CAAACAATTAAATCCAACACAACATTGGAACAAAGTTAAATTAAAGGATTTTTAGCTGTT
GTTGAAGAACTCTTGTGGGATTACGGATGGACAAAACTTCTTCGCCACAAACTTGTACTG
CAAAAAAAAAACACAAAAATCCAATCGTTATTAGCACATAAAACAACTTAATCATCATTC
TTAATTGTTATCACAAATAAATAAACACAACACCTTACTTTGTGTTGACTATACTTCTCT
CTAACAGCAGGTAGTGTAAAGCCGTGAGCAGTACTACACAATCGTTGAAGATCCTTCACA
CATCCTCTCAACTCCATCGCTTTATATGGCGTATAGTGTTGCAACGTCAAGTTCTGATCC
AACCAAACAACAAACACATTTTACTACATGATTCTTATAAAGAGTAAAACAGTGTCTTAA
GGTTGTTTAAACATACCCATGGTCGTCTTGTTGGGTCTAGAAGGTACTTAGCCAAGAAAA
TGGC
   
Sequence Name 22-K119069-0022-011-E09C-3144
GenBank Accession LN496334 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K119069-0022-011-E09C-3144
CTTCCTCTATATAGGAAGTTCATTTCATTTGGAGCGGTACAGGGTACCCGGGGATCAGAT
TGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGTCGAAGTTGGATCGTACAACTT
GAATTTTCAGACGCTTTGTTCGAGCTGCTCATGGTGTTCATGAAGCTCCATTGATGCAGC
TAGAATGTATGGCTAACTACATTGCGGAATTATCGCTACTAGAGTACACTATGCTCTCTC
ATTCTCCTTCACTAGTAGCTGCTTCCGCCATTTTCTTGGCTAAGTACATTCTAGACCCAA
CAAGACGACCATGGGTATGTTTAAACAACCTTAAGACACTGTTTTACTCTTTATAAGAAT
CATGTAGTAAAATGTGTTTGTTGTTTGGTTGGATCAGAACTCGACGTTGCAACACTATAC
GCAATATAAAGCGATGGAGTTGAGAGGATGTGTGAAGGATCTTCAACGATTGTGTAGTAC
TGCTCACGGCTCTACACTACCTGCTGTTAGAGAGAAGTATAGTCAACACAAAGTAAGTTG
TTGTGTTTATTTATTTGTGATAACAATTAAGAATGATGATTAAGTTGTTTTATGTGCTAA
TAACGATTGGATTTTTGTGTTTTTTTTTTGCAGTACAAGTTTGTGGCGAAGAAGTTTTGT
CCATCCGTAATCCCACAAGAGTTCTTCAACAACAGCTAAAAATCCTTTAATTTAACTTTG
TTCCAATGTTGTGTTGGATTTAATTGTTTGATTGTACATGATTTTGCTGTTGTTGGTCTA
TTTATAAAATTAGCTATCTACAATCTCACTTTGAAAACTTGTTAAGCTGATTTATTTTGG
C

Last Updated on 06.12.2018 16:11