Genotyping and primer details


Genotyping and primer details for 011E09 - At1g44110
Line ID 011E09
Locus At1g44110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:16775631 of line 011E09 Chr1:16775631
   
Insertion allele amplicon 3144 - 52xh
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 52xh: 5'-TTGATGTCTTACGGAAATCAAGAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:16774679
   
Insertion allele amplicon 3144 - AS02
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer AS02: 5'-GGAAGCAGCTACTAGTGAAGGAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:16775468
   
wt amplicon (over InsPos) FI02 - AS02
wt amplicon start Chr1:16775468
wt amplicon end Chr1:16776094
wt amplicon size 627 bp
Locus specific primer AS02: 5'-GGAAGCAGCTACTAGTGAAGGAGA-3'
2nd locus specific primer FI02: 5'-TAGTTGATTGGCTTATTGAGGTGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11