SimpleSearch - Line and FST details
Line specific information
| Line ID | 011E09 |
| Vector Used | pAC106 |
| Line Availability | available as T3 set from NASC (N401017) |
| Segregation Analysis | 50:44:32 |
| Confirmed for Hit | At1g44110 |
| Parent of DUPLO pair | 2635 |
| Parent of pair(s) | 348 |
| Note |
Gene hit F15I1
| Sequence (A. th genome BLAST matches underlined) | >54-K028183-022-011-E09t-8409 GGACCGAATTTCGCAAACCGATGCGTGCTATGGGATCCTCCCTATAGTGAGTCGTATTAC TCACTCTCATGTAAAATTACCTTTGCCGACATGTCTGCAAACGTGAATGAAAAACTCCNG AAAATTATTTTGTCAAATTGTAGGGAAACAANATTTTNAATCTTGAGTGGGATGANNATA GGTGGGGGGGGGCAANAACCAAAAACTCTTAAAAAATCTACAAGACATTCAAGGCCGTTG AGAACTGTAAAGAGAAAAGAAGAGTTGAAAAAGATAAGAAAACA |
| GenBank Accession | CR769873 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr1:20208109 - go to primer design |
| BLAST e Value | 2e-17 |
| Hit Clone Code (BAC ID) | F15I1 |
| Insertion Classification | Genome Hit |
| Confirmation Status | unknown |
| Other FSTs Supporting this Hit | AL935980 [GenBank] |
Gene hit At5g66710
| Sequence (A. th genome BLAST matches underlined) | >69-K013043-022-011-E09-35St AAATGGACGTAAGGGATCGACGCTAATCCCACTATCCTTCGCAAGACCCTTCCTCTATAT AAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCGGGGATCAGATTGTCGTTTCCCG CCTTCGGTTTAAACTATCAGTGTTTGTCGAAGTTGGATCGTACAACTTGAATTTTCAGAC GCTTTGTTCGAGCTGCTCATGGTGTTCATGAAGCTCCATTGATGCAGCTATGGGATCCTC CCTATAGCGAGTCGATNTACTCACCTCTGATGTACGGTCTGTTATTCTGTGTCTACATCC TTATATCGTCGGTCGGGGATGGTGTAATATCCCTAATGGGATCCTTTTGATGACAGTTGA TTGGGGCGTGCATACAACCGAAGTTGATGATAATTACTGAACTCATGGAAGGCAATACTC TTCACAAGTTTATGTTGAGTGTTCGTCCAAAGCCTCTTGATCTGAGAGCTCTCCATTAGC TTTGCGTTGGATATTGCTCGGGGAATGGAGTTCTTGAATGCCAATGGCATTATTCACCGA GATTTGAAACCCAAGTGAGTGAAACTGAGACCTCCCCGAACCGAGGATTCAACATCTGTG AATGGAATAAGAATGGTTTTTGGGCAGGTAAAATGGTCTTAACAGGTGATCATAAGCATC GTAAAGTTG |
| GenBank Accession | BX890649 [GenBank] |
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| Predicted Position of Insertion | Chr5:26637327 - go to primer design |
| BLAST e Value | 1e-127 |
| Hit Clone Code (BAC ID) | MSN2 |
| Hit Gene Code | At5g66710 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | Protein kinase superfamily protein |
| Insertion Classification | CDSi |
| Confirmation Status | unknown |
Gene hit T23E18
| Sequence (A. th genome BLAST matches underlined) | >32-K016981w-022-011-E09T-8409 TGATCTGAAATTTCCCGGACATGAAGCCATTTACTACAATTATGAAATAAGAGAATAGAC GATTTACTAATAATTATTTGTCTAACAAAATAATAATTGTCTACAAAATTATTAAGAAAT CAAAATTATTATAATCGTCTGTAATTATTTGTGAATGAAAAACTCCAACAAAATTATTTT TTTAAATTGTAGGGAAACCAAAATTTTAAAATCTTGATTGGTATGAAAATAAGTTTTTGA AAAACAAAAACCAAAAACTCTTAAAAAATCTACAAAACATTCAAGGCCGTTGATAACTGT ATACTAAAATACTATGGGATCCTCCCCNTATAGTGAGATAAACCTCTTAAAGAATTAGAA GGCCTATGGGATCCCCCNTATAGTGAGA |
| GenBank Accession | AL935981 [GenBank] |
| Graphic View |
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| Predicted Position of Insertion | Chr1:28592877 - go to primer design |
| BLAST e Value | 7e-53 |
| Hit Clone Code (BAC ID) | T23E18 |
| Insertion Classification | Genome Hit |
| Confirmation Status | unknown |
Gene hit At1g05570
| Sequence (A. th genome BLAST matches underlined) | >69-K012256-022-011-E09-8409 TCCGTCTATTCGCGTCGCCTATTTTGATGAGGACGAGCACTCACGTAAAGAAAGTTATAA GGGGACCGAGGAGAAAATTTATTACTCGGCGCTTGTAAAAGCTGCTCCACAGACCAAACC AATGGATTCTTCTGAATCTGTTCACACACTATGGGATCCTCCCTAGAGTGAATCATATTA CTCAGAGAAACGTATGCCCCACTCTCTTACATTTTCTGCATGGCATTCAAGCTCGGGGAT AGGAGATTATAAAGCACTAGGAGAGGACTAATAGTATTTATGTCTCCAAAGGATGAGAGT GAACACTTTCGTAGGTCGTTACCTATGGGATCCTCCCTATAGTGAGTCGTTTTACTCGAA TGTTTGGNCGGGGCTTGGCAACGGGATGGATCATGGAAAGAAAGGAACTATGGGATCCTC CCTATAGGGAGTCGGATAAACGAAGGATTCACCGAGCAGGGGGGACCCCCTTAAAAGGGG ACCCCGTGGTCTGGGCCCGGGGGGGCCGCGTTTCCCAGAGCGGCGGAATAAAATTTGGGC CCCTTGGCCCCTAAAAACCCTATCTAGGGGGGGTCGCCCCCCCAAAGCCTTATAGAGGAA AACATTTATTGCGGCGCCCCTCGGGGGGAGAAACCNCAACCATCCCAGAAGGGGGCCAGA ATTCCGGGAGTACAAAAGGGGGAGTNCCCGCCGTCTTTTTGCGGGAAAAAAACACAAAAA TNNCACACCTCTTTTTTCACCCAAAAGAGGGGGAGAGACCCCGGGTCGTGCTAAAAAACA AGAGCATCGTTGTTTTTACAAGAACACCGCCCCCCCCCGTCCTTTTTGGGCGGAGGGGCG TATAAAATTGTTCTTTCTCTGGAGAAGGTTTGTTTC |
| GenBank Accession | AL752229 [GenBank] |
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| Predicted Position of Insertion | Chr1:1650948 - go to primer design |
| BLAST e Value | 1e-53 |
| Hit Clone Code (BAC ID) | T25N20 |
| Hit Gene Code | At1g05570 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | callose synthase 1 |
| Insertion Classification | CDSi |
| Confirmation Status | failed, line belongs to a contamination group, please check line 013E09 |
| Other FSTs Supporting this Hit | AL752231 [GenBank] AL752230 [GenBank] |
Gene hit At1g44110
| Sequence (A. th genome BLAST matches underlined) | >69-K013044-022-011-E09-35St TTGCGTAAGGGATGACGCACAATCCCCTATCCTTCGCAGACCCTTCCTCTATATAAGGAA GTTCATTTCATTTGGAGAGGACAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCG GTTTAAACTATCAGTGTTTGTCGAAGTTGGATCGTACAACTTGAATTTTCAGACGCTTTG TTCGAGCTGCTCATGGTGTTCATGAAGCTCCATTGATGCAGCTATGGGATCCTCCCTATA GTGAGTCGTTTTACTCACTACTATGGGACCCTACGTATTCTGATTCTGCTTACTTATGGG ATGCTCCCTCCATTGACTTGGCTTACTCCNTTCTATGGGATCCTCCCTATAGTGAGTCGT ATTTACTC |
| GenBank Accession | BX890650 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr1:16775631 - go to primer design |
| BLAST e Value | 1e-37 |
| Hit Clone Code (BAC ID) | T7O23 |
| Hit Gene Code | At1g44110 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | Cyclin A1;1 |
| Insertion Classification | CDSi |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
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Last Updated on 10.06.2021 13:37 |
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