Sequences from confirmation amplicon


Confirmation sequences for line 011F11 and hit At3g14270 are shown.
Line ID 011F11
Locus At3g14270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4756440
Primers used for PCR 01vh - 3144, show primer details
   
Sequence Name 70-K117751-0022-011-F11C-01vh
GenBank Accession LN512456 [GenBank]
Sequencing primer gene specific primer, 01vh: 5'-GTGAATCTTAGCAACAGCCATCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K117751-0022-011-F11C-01vh
GGTTTAGACATAGATAACAAAATCCATTTCTAACCACCAACAGGAAGAAAAAGAAATATG
TAAGCGGCAGGCAAATTTCACCTGTTGCAACAAAGTATCAAAACTTGATAACTGGTTTGA
GACCCTTTGATACTCAAGTCCACCGCCTAGAATCAATAGTCGAGCTTTCTGATTCAAACA
CTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTC
CAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGC
GTCATCCCTTACGTCAGTGGAGATATCACATCTCAATCCA
   
Sequence Name 45-K117750-0022-011-F11C-35St
GenBank Accession LN505671 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K117750-0022-011-F11C-35St
ACCCTTCCTCTATATAAGGAAGTTTCATTTCATTTGGAGAGGACAGGGTACCCGGGGATC
AGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGAATCAGAAAGCTCGACTA
TTGATTCTAGGCGGTGGACTTGAGTATCAAAGGGTCTCAAACCAGTTATCAAGTTTTGAT
ACTTTGTTGCAACAGGTGAAATTTGCCTGCCGCTTACATATTTCTTTTTCTTCCTGTTGG
TGGTTAGAAATGGATTTTGTTATCTATGTCTAAACCCTTCTACCTCTTAAACATGTTTTG
GTTTTCAGGAAAAGGATCATCTAAAGATGGCTGTTGCTAAAATTCAC

Last Updated on 06.12.2018 16:11