Genotyping and primer details


Genotyping and primer details for 011F11 - At3g14270
Line ID 011F11
Locus At3g14270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:4756440 of line 011F11 Chr3:4756440
   
Insertion allele amplicon 3144 - 01vh
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 01vh: 5'-GTGAATCTTAGCAACAGCCATCTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:4756680
   
wt amplicon (over InsPos) 66vo - 01vh
wt amplicon start Chr3:4755987
wt amplicon end Chr3:4756680
wt amplicon size 694 bp
Locus specific primer 01vh: 5'-GTGAATCTTAGCAACAGCCATCTT-3'
2nd locus specific primer 66vo: 5'-GCACAAGAAGGCTATGAAGAATGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11