Sequences from confirmation amplicon


Confirmation sequences for line 011H05 and hit At3g11640 are shown.
Line ID 011H05
Locus At3g11640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3674255
Primers used for PCR PD20 - 3144, show primer details
   
Sequence Name 28-K036416-022-011-H05C-PD20
GenBank Accession LN498749 [GenBank]
Sequencing primer gene specific primer, PD20: 5'-GAGAACCCTCCAGGTAAAGATACA-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K036416-022-011-H05C-PD20
CCGCTCTTTCCTTTCCTCGATTTCCGGTATGCTTAATACGTTTTCAATGTTGCAGGCATT
ACAGTACATCCAGGAAAACCCGGATGAAGTCTGCCCAGCAGGATGGAAGCCGGGTGAGAA
GTCAATGAAACCCGACCCAAAACTCAGCAAAGAGTACTTCTCAGCTATTTAGAAACTCTA
CTATGATAGCAAAAAGGTACAATCTTTGTTATATGTGAGCAGAGTTTTTTTTCTTGTACG
CTAAAACAATCCTTTGTTTGATTCTCACTTTGTCCCCAAAATTATAATAAAAAACTTTTT
CCGTTAATGGAAGTCTTATTGTGTTACGCCTACAAATTCATATTCAGTTGATCACTTACC
TAAGCATGTACACTGTACATATTAATGGTAAAAATCAAGACCATTTTGTGATCTTCTTTG
ATAGCACAGAGAAGCCATTGTTCTACCATATACGCTAGTCATATTAAAGGACCTGTAAAA
AAACCAAAGGCTAACTACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCC
CCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCG
AAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAATCCACTT
GCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCCACGATGCTCCTCGTGGGTGG
   
Sequence Name 20-K036416-022-011-H05C-3144
GenBank Accession LN495124 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K036416-022-011-H05C-3144
ATCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAG
GACAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTAG
TTAGCCTTTGGTTTTTTTACAGGTCCTTTAATATGACTAGCGTATATGGTAGAACAATGG
CTTCTCTGTGCTATCAAAGAAGATCACAAAATGGTCTTGATTTTTACCATTAATATGTAC
AGTGTACATGCTTAGGTAAGTGATCAACTGAATATGAATTTGTAGGCGTAACACAATAAG
ACTTCCATTAACGGAAAAAGTTTTTTATTATAATTTTGGGGACAAAGTGAGAATCAAACA
AAGGATTGTTTTAGCGTACAAGAAAAAAAACTCTGCTCACATATAACAAAGATTGTACCT
TTTTGCTATCATAGTAGAGTTTCTAAATAGCTGAGAAGTACTCTTTGCTGAGTTTTGGGT
CGGGTTTCATTGACTTCTCACCCGGCTTCCATCCTGCTGGGCAGACTTCATCCGGGTTTT
CCTGGATGTACTGTAATGCCTGCAACATTGAAAACGTATTAAGCATACCGGAAATCGAGT
GAAAGGAAAGAGCGGTTTTTGGAGTTGTGTGTATTTATTTGTATCTTTACCTGGAGGGT

Last Updated on 06.12.2018 16:11