Genotyping and primer details


Genotyping and primer details for 011H05 - At3g11640
Line ID 011H05
Locus At3g11640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:3674255 of line 011H05 Chr3:3674255
   
Insertion allele amplicon 3144 - PD20
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer PD20: 5'-GAGAACCCTCCAGGTAAAGATACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:3673717
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11