Sequences from confirmation amplicon


Confirmation sequences for line 017F08 and hit At3g11270 are shown.
Line ID 017F08
Locus At3g11270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3530846
Primers used for PCR 02gz - 8474, show primer details
   
Sequence Name 67-K113077-0022-017-F08C-02gz
GenBank Accession LN511849 [GenBank]
Sequencing primer gene specific primer, 02gz: 5'-AGGGATGTAAAAGACACAACCATC-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K113077-0022-017-F08C-02gz
AACTTAAACATTTTCATCATCAAGCTTCTTAATGTTTATTCAAATCATGATTGTTGCTCT
CAAAACAAAATGTGAGGAACATATATTGAGGAAACTGAAATTATTTGTGAACTGTATTTC
AGGTCACTGCGAAACTTACTGCTCTGAAGGGATTGGATGCACGACTTCGGGAGATCCGGA
CTTACCTTGACCTTGTAATCGAAGGAAAACTCCCATTAAACCATGAAATCTTATACCATC
TTCAGGTAACACTTTTAATTGTAGTTGACTAGTAGAATCAAACTGGTTTTCGTGTTTGTT
TTTTTCTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATTC
   
Sequence Name 40-K113075-0022-017-F08C-8409
GenBank Accession LN503766 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K113075-0022-017-F08C-8409
ATTGAATATATCCTGAGAAAAAACAACACGAAAACCAGTTTGATTCTACTAGTCAACTAC
AATTAAAAGTGTTACCTGAAGATGGTATAAGATTTCATGGTTTAATGGGAGTTTTCCTTC
GATTACAAGGTCAAGGTAAGTCCGGATCTCCCGAAGTCGTGCATCCAATCCCTTCAGAGC
AGTAAGTTTCGCAGTGACCTGAAATACAGTTCACAAATAATTTCAGTTTCCTCAATATAT
GTTCCTCACATTTTGTTTTGAGAGCAACAATCATGATTTGAATAAACATTAAGAAGCTTG
ATGATGAAATGTTTAAGTTATGTGTGAAGAAAATAACCTCAGTTGCCAAGGTACTGATGG
TTGTG

Last Updated on 06.12.2018 16:11