Genotyping and primer details


Genotyping and primer details for 017F08 - At3g11270
Line ID 017F08
Locus At3g11270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:3530846 of line 017F08 Chr3:3530846
   
Insertion allele amplicon 8474 - 02gz
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 02gz: 5'-AGGGATGTAAAAGACACAACCATC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:3530478
   
wt amplicon (over InsPos) 01jz - 02gz
wt amplicon start Chr3:3530478
wt amplicon end Chr3:3531329
wt amplicon size 852 bp
Locus specific primer 02gz: 5'-AGGGATGTAAAAGACACAACCATC-3'
2nd locus specific primer 01jz: 5'-ATTTGGCTGCATTTAACTCTCAGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11