Sequences from confirmation amplicon


Confirmation sequences for line 021D08 and hit At1g64180 are shown.
Line ID 021D08
Locus At1g64180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:23821996
Primers used for PCR 01ra - 8474, show primer details
   
Sequence Name 69-K115895-0022-021-D08C-01ra
GenBank Accession LN512266 [GenBank]
Sequencing primer gene specific primer, 01ra: 5'-TCTGCAGGACTAAGAAACCAAAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K115895-0022-021-D08C-01ra
GCCTTCAGCTTGCGAGGTTCGTGTCTCCCTTAAAGGATTAAGGGCTTTGGTGTGGGTGAT
TTGAGCGAAATAAATCAGCTAAATAAACAAAAGTTAACTCTCTCCAACCAATGACTTGCA
AAATTCTTAACAAAGAACAGAGCAATTGGGTAAACCTAGAACACTTATCACCAATATGAG
AATCCAAATTTGGCCAAAACCCTGAACCAGAAACAGAAACAAAACAATCTAGGGCTAAGA
CATTCAGAAATTCATCACAACCCATGTCACAAAATTCAAACTTTAAAAGGCAAAACGATA
CATTGTCACAAGATATTCAAACTTGAGCAATGCCCAATCCTAAAACCAATCAGCTTCATG
GGATAATCAAACGAAAAAAAACAAAATTAAATCAAGGCAAAATTACCAGAAAAGGGTAAA
AATCCAATCTCTAGAAAGAGAAGGATAGAGAGAGACCTGGTGATCAGTGAGCTCAATACC
ATTATTGTCTGCAACCGCCGCCTTTCCATGGCCGTGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCGCAGCGTTATT
   
Sequence Name 39-K115894-0022-021-D08C-8409
GenBank Accession LN503477 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K115894-0022-021-D08C-8409
CAATAATGGTATTGAGCTCACTGATCACCAGGTCTCTCTCTATCCTTCTCTTTCTAGAGA
TTGGATTTTTACCCTTTTCTGGTAATTTTGCCTTGATTTAATTTTGTTTTTTTTCGTTTG
ATTATCCCATGAAGCTGATTGGTTTTAGGATTGGGCATTGCTCAAGTTTGAATATCTTGT
GACAATGTATCGTTTTGCCTTTTAAAGTTTGAATTTTGTGACATGGGTTGTGATGAATTT
CTGAATGTCTTAGCCCTAGATTGTTTTGTTTCTGTTTCTGGTTCAGGGTTTTGGCCAAAT
TTGGATTCTCATATTGGTGATAAGTGTTCTAGGTTTACCCAATTGCTCTGTTCTTTGTTA
AGAATTTTGCAAGTCATTGGTTGGAGAGAGTTAACTTTTGTTTATTTAGCTGATTTTTTT
CGCTCAAATCACCCACACCAAAGCCCTTAATCCGATTCGCCAGACACGAACCTCGCAAGC
TGAAGGCAATACTATTGCCATTGATTCTGAGCTCATTTGTGTTTGGTTCTAAGTTACTAG
ATTTTGGTTTCTTAGTCCTGCAG

Last Updated on 06.12.2018 16:11