Genotyping and primer details


Genotyping and primer details for 021D08 - At1g64180
Line ID 021D08
Locus At1g64180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:23821996 of line 021D08 Chr1:23821996
   
Insertion allele amplicon 8474 - 01ra
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 01ra: 5'-TCTGCAGGACTAAGAAACCAAAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:23822559
   
wt amplicon (over InsPos) 01pm - 01ra
wt amplicon start Chr1:23821521
wt amplicon end Chr1:23822559
wt amplicon size 1038 bp
Locus specific primer 01ra: 5'-TCTGCAGGACTAAGAAACCAAAAT-3'
2nd locus specific primer 01pm: 5'-AAACGAGAGAGACAACAGTTCCAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11