Sequences from confirmation amplicon


Confirmation sequences for line 025F04 and hit At4g03110 are shown.
Line ID 025F04
Locus At4g03110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:1378402
Primers used for PCR CD13 - 8474, show primer details
   
Sequence Name 11-K029488-022-025-F04C-8409
GenBank Accession LN490981 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K029488-022-025-F04C-8409
ACTTTTGGTTACTCTTTAATAGAAGCAAAATGTTAGTTTGATCCTTGTTCTTATGGAAAC
CTGAAAAATGTTGGCATTAGTGTTTTTTTACCATGGTTGTTTCAGTGTGTTCTTCGGCCT
TTTGACTGTAACATTTAGGATGCTTAGATCCTTGTCAGGTCTCTAACTAACTTTTTCTTT
TGGTTGCTCAGGCTAACAGTCTATTGCAAGTAAAGTATGCAGATGGCGAATTAGAAAGGC
TAGGTAACGCGATTCCGTGCTCTATTTCACCCATTTTCATTGTTTTATCATCAACTGATG
GATGTTGTATTAATGTATTCTGCAATCCAGAGCATAAGCTATTTGTTGGTATGCTTCCAA
AGAATGTCTCTGAAGCTGAAGTTCAATCTTTATTCTCCAAGTACGGGACCATAAAGGATC
TACAGATTCTAAGAGGTGCTCAACAAACGAGCAAAGGTTGAATCTATCTCTGTGTCGCTA
TTGTGATCTTTGGTTTCACTAGTTTGCTTTACCTAGTTTTGACCTTTTGTTTGTTGTCGA
TGTTTAGGCTGTGCATTTCTGAAGTATGAGACAAAAGAACAAGCTGTTTCCGCCATGGAA
TCTATCAACGGAA
   
Sequence Name 12-K029488-022-025-F04C-CD13
GenBank Accession LN491441 [GenBank]
Sequencing primer gene specific primer, CD13: 5'-CTCCATTTTATGTTTTCCGTTGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K029488-022-025-F04C-CD13
TCTTTTGTCTATACTTCAGAAATGCACAGCCTAAACATCGACAACAAACAAAAGGTCAAA
ACTAGGTAAAGCAAACTAGTGAAACCAAAGATCACAATAGCGACACAGAGATAGATTCAA
CCTTTGCTCGTTTGTTGAGCACCTCTTAGAATCTGTAGATCCTTTATGGTCCCGTACTTG
GAGAATAAAGATTGAACTTCAGCTTCAGAGACATTCTTTGGAAGCATACCAACAAATAGC
TTATGCTCTGGATTGCAGAATACATTAATACAACATCCATCAGTTGATGATAAAACAATG
AAAATGGGTGAAATAGAGCACGGAATCGCGTTACCTAGCCTTTCTAATTCGCCATCTGCA
TACTTTACTTGCAATAGACTGTTAGCCTGAGCAACCAAAAGAAAAAGTTAGTTAGAGACC
TGACAAGGATCTAAGCATCCTAAATGTTACAGTCAAAAGGCCGAAGAACACACTGAAACA
ACCATGGTAAAAAAACACTAATGCCAACATTTTTCAGGTTTCCATAAGAACAAGGATCAA
ACTAACATTTTGCTTCTATTAAAGAGTAAACCAAAAGTGTTTCTTTCGCATTAATCTCAA
GAATGCGATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAA
ACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTC
GGAAATCTTTATNTCGACGTGTCT

Last Updated on 06.12.2018 16:11