Genotyping and primer details


Genotyping and primer details for 025F04 - At4g03110
Line ID 025F04
Locus At4g03110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:1378402 of line 025F04 Chr4:1378402
   
Insertion allele amplicon 8474 - CD13
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CD13: 5'-CTCCATTTTATGTTTTCCGTTGAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:1377775
   
wt amplicon (over InsPos) 08sl - CD13
wt amplicon start Chr4:1377775
wt amplicon end Chr4:1378805
wt amplicon size 1031 bp
Locus specific primer CD13: 5'-CTCCATTTTATGTTTTCCGTTGAT-3'
2nd locus specific primer 08sl: 5'-TTTGTTCTTCTTTCAGATGCTTGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11