Sequences from confirmation amplicon


Confirmation sequences for line 028G12 and hit At1g20200 are shown.
Line ID 028G12
Locus At1g20200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7001724
Primers used for PCR 05ne - 8474, show primer details
   
Sequence Name 30-K114376-0022-028-G12C-05ne
GenBank Accession LN499821 [GenBank]
Sequencing primer gene specific primer, 05ne: 5'-AAATCTCCCAAACACAATTAAACG-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K114376-0022-028-G12C-05ne
ACACAGATAATATCTTTTCCAAAGTGAAACCAGAGTAATAAAAAGGTTCCTGAGGATAAA
AATCCTCAATAAAGAAACACTTAGAGACGATGAGAAGAGTACCAAGTAAACTTGTGAACA
TAAAAAAAGTGAGACAGATGGAGAGTCAACTCTTATAAGAAATAGATGACCTTTGTCTAA
AAATCATCATCGTCTTCCTCAGCCATATGCTTAGCAAGCTCTTCTTCCTGTTGCTTCCTC
TCTCTCCTCTTCTCATCGCTTTCTTTCTCCTTGTGAGTGTTAGGAGGAAACCTCAATGCT
CTGACAGCTTCGTTATGCATGTTGAGGCAGAAAGCAATTCTTGAGTTGAACGCAGTTTGT
GGCTCATTCGTCGAGTAGATGTCCCCAGTTTCTTTGGAGACCATGCATCCGTTTTTGTGA
TCGATTGTAGCATCAATAGCTCCGTCGCGTATGGCCTTTGCCACGATGCTTTCCGCATCA
GCCACAGGGTTTTCAGCCACAGGGTTTTCAGGATATATTCAATTGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCANAAATGTAGATGTCCGCA
   
Sequence Name 16-K114375-0022-028-G12C-8409
GenBank Accession LN493522 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K114375-0022-028-G12C-8409
ATTTCATTGAATATATCCTGAAACCCTGTGGCTGAAACCCTGTGGCTGATGCGGAAAGCA
TCGTGGCAAAGGCCATACGCGACGGAGCTATTGATGCTACAATCGATCACAAAAACGGAT
GCATGGTCTCCAAAGAAACTGGGGACATCTACTCGACGAATGAGCCACAAACTGCGTTCA
ACTCAAGAATTGCTTTCTGCCTCAACATGCATAACGAAGCTGTCAGAGCATTGAGGTTTC
CTCCTAACACTCACAAGGAGAAAGAAAGCGATGAGAAGAGGAGAGAGAGGAAGCAACAGG
AAGAAGAGCTTGCTAAGCATATGGCTGAGGAAGACGATGATGATTTTTAGACAAAGGTCA
TCTATTTCTTATAAGAGTTGACTCTCCATCTGTCTCACTTTTTTTATGTTCACAAGTTTA
CTTGGTACTCTTCTCATCGTCTCTAAGTGTTTCTTTATTGAGGATTTTTATCCTCAGGAA
CCTTTTTATTACTCTGGTTTCACTTTAGGAAAAGATATTTATCTTGTTGGTTTCAGAAGA
TCAAATTCAACTTCGTTTAATTGTGTTGGG

Last Updated on 06.12.2018 16:11