Genotyping and primer details


Genotyping and primer details for 028G12 - At1g20200
Line ID 028G12
Locus At1g20200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:7001724 of line 028G12 Chr1:7001724
   
Insertion allele amplicon 8474 - 05ne
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 05ne: 5'-AAATCTCCCAAACACAATTAAACG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:7001182
   
wt amplicon (over InsPos) 14vg - 05ne
wt amplicon start Chr1:7001182
wt amplicon end Chr1:7002063
wt amplicon size 882 bp
Locus specific primer 05ne: 5'-AAATCTCCCAAACACAATTAAACG-3'
2nd locus specific primer 14vg: 5'-TTGTTTTCTGCTCCTTCGAATTAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11