Sequences from confirmation amplicon


Confirmation sequences for line 052A07 and hit At3g03300 are shown.
Line ID 052A07
Locus At3g03300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:771623
Primers used for PCR RE45 - 3144, show primer details
   
Sequence Name 35-K026694-022-052-A07C-3144
GenBank Accession LN501687 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K026694-022-052-A07C-3144
GGATGACGCACAATCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCATT
TCATTTGGAGAGGACAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAA
CTATCAGTGTTTGCAGTTACCATCAATCCCATTGCTGATCTATCTTCGGTTTAGACAATT
GCATCCAACATTTGCAGTTTCATACAGTCTCGTGGGCGTGCTAGAATGCAAAATTCAGAT
TATTTGATGATGGTGGAAAGGTCTGTTACATATTTGGCTGGTCTTCAGAGAGTTTACGAT
TTGTTTTGAAAACAATAGCTCCATGCTTAAAAAAAATATAATTCTTTCTCACTCATTGCC
ACTGGCAGCGGAGATCTGTTAACACAATCTCGATTAATGAAATATCTTTCTGGTGGGAAA
AGAATGCGCGAAGAGTCTTTGGATCATTCTCTTGTTCCCTGTCCACCTCTTCCAGATGAT
TCAGATGAACCACTCTTCCGTGTCGAAAGTACTGGAGCAACTGTAACTCTTAGCTCAAGC
GTCAGCTTAATATATCATTACTGCTCAAGGCTTCCTTCAGATGAGTTAGTCTAATACCAG
CTTCAT
   
Sequence Name 36-K026694-022-052-A07C-RE45
GenBank Accession LN502081 [GenBank]
Sequencing primer gene specific primer, RE45: 5'-GGAAAACTAACTGATGAAGCTGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K026694-022-052-A07C-RE45
AACTCATCTGAAGGAAGCCTTGAGCAGTAATGATATATTAAGCTGACGCTTGAGCTAAGA
GTTACAGTTGCTCCAGTACTTTCGACACGGAAGAGTGGTTCATCTGAATCATCTGGAAGA
GGTGGACAGGGAACAAGAGAATGATCCAAAGACTCTTCGCGCATTCTTTTCCCACCAGAA
AGATATTTCATTAATCGAGATTGTGTTAACAGATCTCCGCTGCCAGTGGCAATGAGTGAG
AAAGAATTATATTTTTTTTAAGCATGGAGCTATTGTTTTCAAAACAAATCGTAAACTCTC
TGAAGACCAGCCAAATATGTAACAGACCTTTCCACCATCATCAAATAATCTGAATTTTGC
ATTCTAGCACGCCCACGAGACTGTATGAAACTGCAAATGTTGGATGCAATTGTCTAAACC
GAAGATAGATCAGCAATGGGATTGATGGTAACTGCAAACACTGATAGTTTAAACCGAAGG
CGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTT
ATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGG
AGATATCACATCAATCCACTTGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCCACGA
TGCTCCT

Last Updated on 06.12.2018 16:11