Genotyping and primer details


Genotyping and primer details for 052A07 - At3g03300
Line ID 052A07
Locus At3g03300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:771623 of line 052A07 Chr3:771623
   
Insertion allele amplicon 3144 - RE45
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer RE45: 5'-GGAAAACTAACTGATGAAGCTGGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:771184
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11