Sequences from confirmation amplicon


Confirmation sequences for line 054B03 and hit At5g04040 are shown.
Line ID 054B03
Locus At5g04040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1090694
Primers used for PCR 07kk - 8474, show primer details
   
Sequence Name 63-K113595-0022-054-B03C-07kk
GenBank Accession LN511031 [GenBank]
Sequencing primer gene specific primer, 07kk: 5'-AGGTAAAAGCTTATGCTCAACCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K113595-0022-054-B03C-07kk
AGAGCCCCACCACTCAAAGCAAAGCCGTTCTACCAAAGGCATGCCGTGTTTCATGCATAA
AAGAAAGCTTCTCTTCTAAAGAAAGCTCCTCTGAATCAGAGTTACAAACCATTCTCAACT
GAGTAGACACCTCATCAATGTACTCTTTGATATGTCTAGGAACCTGAAGTCTACCTTTAT
GAAGCTCCGAATTACACATATTACCGAGATTCCTCACCAAATCAGCTCTCATACAAAACA
TAATGTCTCTAAGTGAGCCTTCTTGGCGACGATGACGAAGCTCCTGAAGCTTGTTCTTAA
CCAACTCTTCATCATAAAGATCAGATTCATTCATCTTTGGTGTTTCCTTCTCTAACATCT
TAGCAGCGGCGGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 21-K113594-0022-054-B03C-8409
GenBank Accession LN495743 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K113594-0022-054-B03C-8409
ATCCTGCCGCCGCTGCTAAGATGTTAGAGAAGGAAACACCAAAGATGAATGAATCTGATC
TTTATGATGAAGAGTTGGTTAAGAACAAGCTTCAGGAGCTTCGTCATCGTCGCCAAGAAG
GCTCACTTAGAGACATTATGTTTTGTATGAGAGCTGATTTGGTGAGGAATCTCGGTAATA
TGTGTAATTCGGAGCTTCATAAAGGTAGACTTCAGGTTCCTAGACATATCAAAGAGTACA
TTGATGAGGTGTCTACTCAGTTGAGAATGGTTTGTAACTCTGATTCAGAGGAGCTTTCTT
TAGAAGAGAAGCTTTCTTTTATGCATGAAACACGGCATGCCTTTGGTAGAACGGCTTTGC
TTTTGAGTGGTGGGGCTTCTCTTGGTGCGTTTCATGTTGGTGTGGTTAGGACTTTGGTTG
AGCATAACNTTTTACCTA

Last Updated on 06.12.2018 16:11