Genotyping and primer details


Genotyping and primer details for 054B03 - At5g04040
Line ID 054B03
Locus At5g04040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:1090694 of line 054B03 Chr5:1090694
   
Insertion allele amplicon 8474 - 07kk
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 07kk: 5'-AGGTAAAAGCTTATGCTCAACCAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:1091119
   
wt amplicon (over InsPos) 05lb - 07kk
wt amplicon start Chr5:1090378
wt amplicon end Chr5:1091119
wt amplicon size 742 bp
Locus specific primer 07kk: 5'-AGGTAAAAGCTTATGCTCAACCAA-3'
2nd locus specific primer 05lb: 5'-CTTTTCGATTGGACCATCATCTAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11