SimpleSearch - Line and FST details
Line specific information
| Line ID | 054B03 |
| Vector Used | pAC161 |
| Line Availability | available as T3 set from NASC (N405103) |
| Segregation Analysis | 50:48:46 |
| Confirmed for Hit | At5g04040 |
| Parent of DUPLO pair | 2759 |
| Parent of pair(s) | 7923 |
| Note |
Gene hit At1g08820
| Sequence (A. th genome BLAST matches underlined) | >18-K012489-022-054-B03-35St NAATTGGACGAAGGGACGACGCTTANTCCCCTATCCTTCGCAAGACCCTTCCTCTATATA AGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCGGGGATCAGATTGTCGTTTCCCGC CTTCGGTTTAAACTATCAGTGTTTGAATCCAGTTTCTTTTGCCATCCTCGATGAATCGTA GCTATCTTTAGTTGCCTTCAAGTCATTAGCAGTATATCGATCTTCCACAAAATCCACTGT GGCCATTTTTGGTGTCTTTAACTCCTTTGCATTGTCGGCTGCCTTTAACTCATCATGTCC AACCATCCTATGGGATCCTCCCTATAGTGANNNNNNNNNNN |
| GenBank Accession | AL754351 [GenBank] |
| Graphic View | 
|
| Predicted Position of Insertion | Chr1:2822453 - go to primer design |
| BLAST e Value | 7e-89 |
| Hit Clone Code (BAC ID) | F22O13 |
| Hit Gene Code | At1g08820 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | vamp/synaptobrevin-associated protein 27-2 |
| Insertion Classification | CDSi |
| Confirmation Status | failed, line belongs to a contamination group, please check line 054C05 |
Gene hit At1g20530
| Sequence (A. th genome BLAST matches underlined) | >18-K012357-022-054-B03-8409 NTGATCCATGGTAGATTTCCCGGACATGAANGCCTTTACAATTGAATATATCGCGACGCT GTGGTTATGTTGCTAGCACAAGACACACGGAACGCGAGAGTCTCGGGGAATCACTGGGAG GACTCGTATAGTAACACGTTTTACGCGCTTAAGAGTCTTCGACGATAATGCTCAGTTACA GACGTTGAGTCCTGTATGAAAACTGATATAGTGAGGATTCTCGCACCTATGTGGACTTCC GAGCTTCATATAGGTATTCTTCACGATGCTACTGGCACTCCTGCGTATAGTGTGGACGCG TACTAAATTATGGCCCACTCTTAAACGGACCTACACTTTCGAATCCTTCGGACATTGCAT AGATTAACAAATAATTCTCCCGTTTTATTCATATAATTACAAACACTGGCTGGCTTGGTT CATGCATGGTTTCCGATAAAGGCCTCCGATTATAATTCAAATGAAGATTGTATGTCGTTT TGGACTGTATACTATAATATCGAAGCAGATCCATAACTGGACCTAATCTATGGGATCCTC CCTATAGTGAGTCGTACTACT |
| GenBank Accession | FR801741 [GenBank] |
| Graphic View | 
|
| Predicted Position of Insertion | Chr1:7108399 - go to primer design |
| BLAST e Value | 6e-40 |
| Hit Clone Code (BAC ID) | F5M15 |
| Hit Gene Code | At1g20530 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | girdin (DUF630 and DUF632) |
| Insertion Classification | CDSi |
| Confirmation Status | unknown |
| Other FSTs Supporting this Hit | AL754348 [GenBank] AL754348 [GenBank] FR801741 [GenBank] |
Gene hit At5g04040
| Sequence (A. th genome BLAST matches underlined) | >18-K012480-022-054-B03-8409 NTGATCCATGGTAGATTTCCCGGACATGAANGCCTTTACAATTGAATATATCCTGCCGCC GCTGCTAAGATGTTAGAGAAGGAAACACCAAAGATGAATGAATCTGATCTTTATGATGAA GAGTTGGTTAAGAACAAGCTTCAGGAGCTTCGTCATCGTCGCCAAGAAGGCTCACTTACA GACATTATGTTTTGTATGAGAGCTGATTTGGTGAGGAATCTCGGTAACTATGTGTAATTC GGAGCTTCATAAAGGCTAGACTTCAGGTTCCTATGGGATCCTCCCTATAGTGACGACCCA TGATAGATGATGGTCCAATCGAAAAGGGATCGACACTATGGGATCCTCCCTATAGTGANN NNNNNNNNNNNNNN |
| GenBank Accession | AL754350 [GenBank] |
| Graphic View | 
|
| Predicted Position of Insertion | Chr5:1090694 - go to primer design |
| BLAST e Value | 5e-105 |
| Hit Clone Code (BAC ID) | F8F6 |
| Hit Gene Code | At5g04040 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | Patatin-like phospholipase family protein |
| Insertion Classification | CDSi |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
Gene hit At5g52045
| Sequence (A. th genome BLAST matches underlined) | >18-K012454-022-054-B03-35St TGACGTAAGGGATGACGCACAATCCCCTATCCTTCGCAGACCCTTCCTCTATATAAGGAA GTTCATTTCATTTGGAGAGGACAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCG GTTTAAACTATCAGTGTTTCCCGGGGATATATTCAATTGAAGATGGCTACATGTACGGGA AATCTACATGGATCAGCAGTGAGTATGATGGACAATATGGAAAAAATGACAGAGTATTTA CCAATTCTTTTTCGATTCAGAACTGTACATGGCCACAACGTGATTATACAATGAAAGAAC ACGATGATAAACCAACAGACGACGATCCGTGCGATTGATAGGCGAAAGCAATAAACATTT TATTCTACTTCTGAAAACTTGATTACAACGGGGCTACATTGACGGCCAAATGGGGACTCA |
| GenBank Accession | FR801742 [GenBank] |
| Graphic View |
|
| Predicted Position of Insertion | Chr5:21131606 - go to primer design |
| BLAST e Value | 0.37 |
| Hit Clone Code (BAC ID) | MSG15 |
| Hit Gene Code | At5g52045 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | hypothetical protein |
| Insertion Classification | CDSi |
| Confirmation Status | unknown |
| Other FSTs Supporting this Hit | FR801742 [GenBank] |
|
Last Updated on 10.06.2021 13:37 |
gabi-kat.de 
