Sequences from confirmation amplicon


Confirmation sequences for line 054H07 and hit At2g28030 are shown.
Line ID 054H07
Locus At2g28030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:11934871
Primers used for PCR CL01 - 3144, show primer details
   
Sequence Name 02-K020608w-022-054-H07C-3144
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K020608w-022-054-H07C-314
GTAAGGGATGACGCACAATCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGT
TCATTTCATTTGGAGAGGACAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGT
TTAAACTATCGCCGTCATATACACTCATATACACTCATGGTTGGTCTAAGCTGGGGACCT
TCATCGCTCATCACTCAGATGGGCGGTGAGTACCCAGGTTTGATGTCTTACTGTTTTGCT
AGTCAAGGAACTAGTAAGATCAATTTTGGAACAAATGCTATTGTTGCAGGAGATGGGGTT
GTATCAACCACTATGTTTCTCACGACGGCGAAACCAGGTTTATATTACCTAAATCTAGAC
GCGGTCAGCGTTGGGGACACCCATGTTGAGACAATGGGGACAACGTTTCATGCGTTAGAA
GGGAACATAATTATAGACTCTGGAACCACTCTAACCTACTTTCCTGTGAGCTACTGCAAC
CTAGTA
   
Sequence Name 10-K020608-022-054-H07C-CL01
GenBank Accession LN490453 [GenBank]
Sequencing primer gene specific primer, CL01: 5'-CTGCCTCTCTTACTAGGTTGCAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K020608-022-054-H07C-CL01
CACAGGAAAGTAGGTTAGAGTGGTTCCAGAGTCTATAATTATGTTCCCTTCTAACGCATG
AAACGTTGTCCCCATTGTCTCAACATGGGTGTCCCCAACGCTGACCGCGTCTAGATTTAG
GTAATATAAACCTGGTTTCGCCGTCGTGAGAAACATAGTGGTTGATACAACCCCATCTCC
TGCAACAATAGCATTTGTTCCAAAATTGATCTTACTAGTTCCTTGACTAGCAAAACAGTA
AGACATCAAACCTGGGTACTCACCGCCCATCTGAGTGATGAGCGATGAAGGTCCCCAGCT
TAGACCAACCATGAGTGTATATGAGTGTATATGACGGCGATAGTTTAAACCGAAGGCGGG
AAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATAT
AGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGAT
ATCACATCAATCCACTTGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCCACGATGCT
CCTCGTGGGTGGG
Primers used for PCR 81wb - 8474, show primer details
   
Sequence Name 22-K118209-0022-054-H07C-81wb
GenBank Accession LN496304 [GenBank]
Sequencing primer gene specific primer, 81wb: 5'-TTTTGAATTTTAGGTTCATCAACAA-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K118209-0022-054-H07C-81wb
ATTTTGCTATAGCAAGGTGATCATCTAATTTCCATTAATACAAAAAGTCAATCTAAAAGA
ATGTAAAACCTAGAGAGAAAAATAAAACTTAGTAGTAACAAAATGTAAAATTCCCCCAAG
TTAGAAGACGTAATGGCAATAACAAAATTGAACGAAGTTGACAAAATTGAAAACTTAACA
ATTACGACAAAAGGTCTAACATGTATAAATATGTGCATGAATTAATGAAGAACATTACAT
TGTGTCTGAAAAACCAAAAATATTATTCGATATCAATCAATGTCTCTTGCAACCACAATG
ATCGTTCTTTTTCTTCAAATCATTACATGTTCTCTCTTCACGACCACTGCCTCATCACCT
CACGGCTTCACCATTGACTTGATCCAGCGTCGTTCGAATTCATCTTCTTCTCGACTGTCC
AAAAATCAGTTGCAAGGAGCATCACCTTACGCCGATACTTTATTTGACTACAACATCTAT
CTAATGAAACTACAAGTCGGTACTCCTCCTTTCGAGATCGAAGCGGAGATAGACACAGGA
AGTGACCTCATATGGACACAATGTATGCCTTGTACTAACTGCTACAGCCAATACGCTCCT
ATATTCGACCCTTCGAATTCTTCAACCTTCAAAGAAAAAAGATGCAACGGGAACTCTTGT
CATTACAAGATTATCTACGCGGACACAACCTATTCCAAGGGAACCTTGGCAACCGAGACG
GTCACGATCCATTCCACTTCAGGGGAACCCTTTGTGATGCCTGAAACCACTATTGGTTGT
GGCCACAACAGCTCATGGTTTAAACCTACTTTTTCGGGCATGGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGNCATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCATTT
   
Sequence Name 10-K118207-0022-054-H07C-8409
GenBank Accession LN490860 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K118207-0022-054-H07C-8409
CCGAAAAGTAGGTTTAAACCATGAGCTGTTGTGGCCACAACCAATAGTGGTTTCAGGCAT
CACAAAGGGTTCCCCTGAAGTGGAATGGATCGTGACCGTCTCGGTTGCCAAGGTTCCCTT
GGAATAGGTTGTGTCCGCGTAGATAATCTTGTAATGACAAGAGTTCCCGTTGCATCTTTT
TTCTTTGAAGGTTGAAGAATTCGAAGGGTCGAATATAGGAGCGTATTGGCTGTAGCAGTT
AGTACAAGGCATACATTGTGTCCATATGAGGTCACTTCCTGTGTCTATCTCCGCTTCGAT
CTCGAAAGGAGGAGTACCGACTTGTAGTTTCATTAGATAGATGTTGTAGTCAAATAAAGT
ATCGGCGTAAGGTGATGCTCCTTGCAACTGATTTTTGGACAGTCGAGAAGAAGATGAATT
CGAACGACGCTGGATCAAGTCAATGGTGAAGCCGTGAGGTGATGAGGCAGTGGTCGTGAA
GAGAGAACATGTAATGATTTGAAGAAAAAGAACGATCATTGTGGTTGCAAGAGACATTGA
TTGATATCGAATAATATTTTTGGTTTTTCAGACACAATGTAATGTTCTTCATTAATTCAT
GCACATATTTATACATGTTAGACCTTTTGTCGTAATTGTTAAGTTTTCAATTTTGTCAAC
TTCGTTCAATTTTGTTATTGCCATTACGTCTTCTAACTTGGGGGAATTTTACATTTTGTT
ACTACTAAGTTTTATTTTTCTCTCTAGGTTTTACATTCTTTTAGATTGACTTTTTGTATT
AATGGAAATTAGATGATCACCTTGCTATAGCAAAATGTATAATTTCTTGGAAATTTTAGT
CTAAAGTTATTGT

Last Updated on 06.12.2018 16:11