Genotyping and primer details


Genotyping and primer details for 054H07 - At2g28030
Line ID 054H07
Locus At2g28030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:11934871 of line 054H07 Chr2:11934871
   
Insertion allele amplicon 8474 - 81wb
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 81wb: 5'-TTTTGAATTTTAGGTTCATCAACAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:11935741
   
Insertion allele amplicon 3144 - CL01
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer CL01: 5'-CTGCCTCTCTTACTAGGTTGCAGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:11934525
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11