SimpleSearch - Line and FST details
Line specific information
| Line ID | 054H07 |
| Vector Used | pAC161 |
| Line Availability | available as T3 set from NASC (N405179) |
| Segregation Analysis | 100:98:94 |
| Confirmed for Hit | At2g17370 At2g28030 |
| Parent of DUPLO pair | none |
| Parent of pair(s) | 9564, 97003 |
Gene hit F24D13
| Sequence (A. th genome BLAST matches underlined) | >56-K012427-022-054-H07-8409 TGATCCATGTAGATTTCCCGGACTTGTGTCTTACTCAGTCATCTGCACTAAAAGTCTAAA GCATAAAATCCTATGGGCTCTCTCTTCTTCTCTCTTTGGGTGCTATTCTATACTACCACT CACCATTTAATACTTGAGACCTAACCTAAGACTATCAGCACTGAAACTACTTTCTAACGG TCTGATGTCACGATAGATTATCTATGGAATCCTTGCTTATTTGGACCATTTTATTGAATT TTTTTTTCTAATTAAGACTAATATTCGCCGAAGTAATTTAAACTGTTCGTTTTGCAAATT TGAGGCTATGGGATCCTCCCTATAGTGAGTCGTATTACTAAACCCCATTGGCTTATTATA TACTTCAAGAGAGTAAAAAATACAGTGAGTCCATGTACTGAATAGAGGCGCGGTGATATG ATCCTTGACGCTGACGACTAAACTGCCTCTTAGGAAAACTCTAACACCATTGCCTCGGTC AATATCACTCCACAAGCGGTTTGGTCGTCACGGCTCTGTTAGCGATTGTTTATGTTACAA CCAGCCGACATCCTCTGTTTGTTCT |
| GenBank Accession | AL754589 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr2:11967782 - go to primer design |
| BLAST e Value | 3e-26 |
| Hit Clone Code (BAC ID) | F24D13 |
| Insertion Classification | Genome Hit |
| Confirmation Status | unknown |
| Other FSTs Supporting this Hit | AL754591 [GenBank] AL754589 [GenBank] |
Gene hit At2g17370
| Sequence (A. th genome BLAST matches underlined) | >56-K012480-022-054-H07-8409 TGATCCATGTAGATTTCCCGGCTTCGTGTATTACTACAGCATCATGCACTAGAAGTCTAG AACATAAAATCCTATGGGATCCTCCCTATAGTGACAAAAACCATAACCCTAAATAATCAT CCCAAAAGTGGAAATAATAAAAGAAGCAGTTAGATTGTGACGGAGGAGACAAAATCATCA TCTCTGTTCTTCTTCTTCTCCTCTCTCACATACGATGATGACCCAACAACCTTGACGTTG ATTTCGATGTCACACACAACACTATAAATAACACTCTTCACCTATTTTTGTCATCTCACA CTTTGGACTTTGGAGCCCAAAATAATCTCATAACCATTAAAAACAAATCTCCTCCTTCTC TCCGGCGTCAATGGAGGATCTCCGTCGTAGATTTCCGACTAAAAAGAACGGAGAAGAAAT CTCTAATGTCGCCGTCGATCCTCCTCTTCGTAAAGCCTCTGACGCGCTTCCTCTTCCGTT ATACCTAACCAACACTTTTTTCCTCTCGCTCTTCTTCGCTACTGTTTATTTTCTTCTTAG CCGATGGCGTGAGAAGATCCGTAACTCGACGCCTCTTCACGTCGTTGACTTATCTGAGAT CTGTGCTCTAATCGGTTTCGTTGCTTCGTTTATCTACCTCCTTGGTTTCTGTGGGATGGA TCTCATTTTCCGATCTTCTTCCGATGATGATNGTTGGGGTAACGATGGGATGATTCCATG TAATCAATCCCTA |
| GenBank Accession | AL754592 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr2:7549695 - go to primer design |
| BLAST e Value | 0.0 |
| Hit Clone Code (BAC ID) | F5J6 |
| Hit Gene Code | At2g17370 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | 3-hydroxy-3-methylglutaryl-CoA reductase 2 |
| Insertion Classification | Promoter |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
Gene hit At2g28030
| Sequence (A. th genome BLAST matches underlined) | >56-K012297-022-054-H07-35St TGACGTAAGGGATGACGCACAATCCCCTATCCTTCGCAGACCCTTCCTCTATATAAGGAA GTTCATTTCATTTGGAGAGGACAGGGTACCCGGGGATCAGACTGTGGTTGACCCTGCTTC GCTTTATACTCTGGGAGTCCTAGTACACTCCTTTCTCTCATGGTTTGACCTTACATGTGA AGACGCTGATTCTCTATGACATCACTATCGTACGGTGAGTACCCATGCTCAATGTCTTAC TGTTTTGCTAGTCAAGGAACTAGTAAGATCAATTTTGGAACAAATGCTATTGTTGCAGGA GATGGGGTTGTATCAACCACTATGTTTCTCACGACGGCGAAACCAGGTTTATATTACCTA AATCTATGGGATCCTACCTATAGCGACTCCATTGACTGAGCGGCGGGAGACGACAAACTT GCGATCGATGCCTCGTATTTATCGACTCTGGAACCACTCTAACTCTACTTTACTGTGAGC TACTGCTGCCTATGGTACACTCCCTTTCGTGAGTACTTCCACGTCAGAGCTACCAATTCA GTGTAGCCCATTACCTTACATAATTCACTCAAATGCTATGGGATCCTCCCTATAGTGAGT CGTTTAACTC |
| GenBank Accession | FR801808 [GenBank] |
| Graphic View |
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| Predicted Position of Insertion | Chr2:11934874 - go to primer design |
| BLAST e Value | 4e-72 |
| Hit Clone Code (BAC ID) | T1E2 |
| Hit Gene Code | At2g28030 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | Eukaryotic aspartyl protease family protein |
| Insertion Classification | CDSi |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
| Other FSTs Supporting this Hit | FR801808 [GenBank] |
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Last Updated on 10.06.2021 13:37 |
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