Sequences from confirmation amplicon


Confirmation sequences for line 055G12 and hit At3g03920 are shown.
Line ID 055G12
Locus At3g03920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1009153
Primers used for PCR PG05 - 8474, show primer details
   
Sequence Name 27-K017447-022-055-G12C-PG05
GenBank Accession LN498166 [GenBank]
Sequencing primer gene specific primer, PG05: 5'-TCACAGGAGAAGATTCCTCATTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K017447-022-055-G12C-PG05
CCGATCTACCTAGAGAACAAGACTCAGATTGGGAAAGTAGATGAAATCTTTGGCCCAATT
AATGAATCTGTAAGTTCTCTATTCTTCTTTCAGTTGTAAGCAAACTAGAGGTTTCTGACA
TTAATTCTGTTTTGATTCTTCTTCAGTTGTTTTCTATCAAAATGATGGAAGGTATTGTAG
CCACCTCGTATTCTCCAGGAGATAAGTTCTTCATCGACCCTTACAAGCTTTTGCCACTCG
CTCGATTCCTTCCTCAGCCAAAGTAAGCCCCACTTCTTAACACATCATTGGTCAATATAT
AAGTTTCTTTTCCTGAACTTGAAATAACATTAGTGAGCTCGTTTAACTTTAGCAATTAGC
TTTTTCCTCTTAACAATCGAGACTTTAGATACACTATTAGTTGAGAGTTGTTAATTGTAT
CAAACTCCATCTAACTGCGTGTCTTTGTTCTTCCAGGGGTCAGTCAACGGGTGGACGTGG
AGGTGCAGGTCGTGGAAGAGGTGATAGTAGAGGTCGTGGAAGAGGTGGATCATTTAGTAG
AGGTAGAGGTGCTCCAAGAGGTGGTAGATTTCCACCACGCGGTGGCTCTCGTTAATATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATG
   
Sequence Name 28-K017447-022-055-G12C-8409
GenBank Accession LN498636 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K017447-022-055-G12C-8409
CATGTAGATTTCCCGGACATATTAACGAGAGCCACCGCGTGGTGGAAATCTACCACCTCT
TGGAGCACCTCTACCTCTACTAAATGATCCACCTCTTCCACGACCTCTACTATCACCTCT
TCCACGACCTGCACCTCCACGTCCACCCGTTGACTGACCCCTGGAAGAACAAAGACACGC
AGTTAGATGGAGTTTGATACAATTAACAACTCTCAACTAATAGTGTATCTAAAGTCTCGA
TTGTTAAGAGGAAAAAGCTAATTGCTAAAGTTAAACGAGCTCACTAATGTTATTTCAAGT
TCAGGAAAAGAAACTTATATATTGACCAATGATGTGTTAAGAAGTGGGGCTTACTTTGGC
TGAGGAAGGAATCGAGCGAGTGGCAAAAGCTTGTAAGGGTCGATGAAGAACTTATCTCCT
GGAGAATACGAGGTGGCTACAATACCTTCCATCATTTTGATAGAAAACAACTGAAGAAGA
ATCAAAACAGAATTAATGTCAGAAACCTCTAGTTTGCTTACAACTGAAAGAAGAATAGAG
AACTTACAGATTCATTAATTGGGCCAAAGATTTCATCTACTTTCCCAATCTGAGTCTTGT
TCTCTAGGTAGATCGGAGCGTTAAAATGAGGAAT

Last Updated on 06.12.2018 16:11