Genotyping and primer details


Genotyping and primer details for 055G12 - At3g03920
Line ID 055G12
Locus At3g03920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:1009153 of line 055G12 Chr3:1009153
   
Insertion allele amplicon 8474 - PG05
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer PG05: 5'-TCACAGGAGAAGATTCCTCATTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:1009774
   
wt amplicon (over InsPos) 02cj - PG05
wt amplicon start Chr3:1008855
wt amplicon end Chr3:1009774
wt amplicon size 920 bp
Locus specific primer PG05: 5'-TCACAGGAGAAGATTCCTCATTTT-3'
2nd locus specific primer 02cj: 5'-CATGGGAAGCTAAAGAACAAGAAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 02.09.2016 15:25