Sequences from confirmation amplicon


Confirmation sequences for line 061E12 and hit At2g43290 are shown.
Line ID 061E12
Locus At2g43290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17992275
Primers used for PCR CK04 - 8474, show primer details
   
Sequence Name 13-K017714-022-061-E12C-CK04
GenBank Accession LN491827 [GenBank]
Sequencing primer gene specific primer, CK04: 5'-TTCTCTTCTGTCAAATCAAACCCT-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K017714-022-061-E12C-CK04
AAGATCAATTACTGCTGCTAAAGCCACCACCTTTCATCATCTGAAGAAACTCTTTGTAAT
TGACTCTACCATCACCATCTGCATCAACTTGCATAATCATCTTCTTACAACCATCTAGGG
TCTTCCCTTGCTTGAGTCCCAAGGAAGCCATCACAGATTTCAACTCCTCCACAGTGATAA
ACCCATCTCCGTCTTGGTCAAACACGTTAAACGCATCTTTCATATCCTCTTCCTCTGTTT
CGCCATCGTTGTGATGCTCATCCACAATCGAGCTGTACAGCGACTCAAACTCGTCTATGT
CGACGCATCCATCACCGTTAGCATCGATCTTGTGGATCATTTGAGTCAGATCTTTGTCTG
GTATGTAGATTCCAAGATTCTCAAGCGAGTCGTTGAGCTCTTCCTTTGTGATTCGACCGT
CACCGTTCTTGTCGAACATCTGGAAAACGCGTTTGAGCTCGGACGGATCTATTCTTTTCG
TCGGCGCTGAAGAAGATGATGGAGAAGGTAACATCGTTGACGGAGACGGTGGTGAGTTCT
TGTGGAGAGTTTTGTCGAACCAAGAAAGAGGGAAAAGAGTTCGTAGCTTCTTTGGTACTA
AAGAGAGAAGAAACGAATTTAGTATATTGTAGAGAAGGAATATTCTCACCATAACTGTTG
AATCACAACTCTTATTTTATGAGAAGAAAAAAACAGAGAGAAGATCTGAGAAACAGAGAG
CTTGA
   
Sequence Name 14-K017714-022-061-E12C-8409
GenBank Accession LN492291 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K017714-022-061-E12C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAAATATTTTTAGAAAAGTCATGTTTCTC
TATAAAATCGTTGTTTTCTTCTTTGTAACGTTCCATTTTAATTTACTTTCTCCAAAAAGT
AAAGGATTAAAAATTAGATTCAACGGTTGTATAGGCGTGAAAAAGAAAAAATAATTTCCG
GAAAAATATACTATTTAATAATTATAATAAATTCTCTCTCTCTCCTCTATAAAGACAAAC
AATCTCCAAGTTTCTCTGTATCAACAAAGCTCTGTTTCAAGCTCTCTGTTTCTCAGATCT
TCTCTCTGTTTTTTTCTTCTCATAAAATAAGAGTTGTGATTCAACAGTTATGGTGAGAAT
ATTCCTTCTCTACAATATACTAAATTCGTTTCTTCTCTCTTTAGTACCAAAGAAGCTACG
AACTCTTTTCCCTCTTTCTTGGTTCGACAAAACTCTCCACAAGAACTCACCACCGTCTCC
GTCAACGATGTTACCTTCTCCATCATCTTCTTCAGCGCCGACGAAAAGAATAGATCCGTC
CGAGCTCAAACGCGTTTTCCAGATGTTCGACAAGAACGGTGACGGTCGAATCACAAAGGA
AGAGCTCAACGACTCGCTTGAGAATCTTGGAATCTACATACCAGACAAAGATCTGACTCA
AATGATCCACAAGATCGATGCTAACGGTGATGGATGCG

Last Updated on 06.12.2018 16:11