Genotyping and primer details


Genotyping and primer details for 061E12 - At2g43290
Line ID 061E12
Locus At2g43290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:17992275 of line 061E12 Chr2:17992275
   
Insertion allele amplicon 8474 - CK04
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CK04: 5'-TTCTCTTCTGTCAAATCAAACCCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:17991275
   
wt amplicon (over InsPos) 17au - CK04
wt amplicon start Chr2:17991275
wt amplicon end Chr2:17992451
wt amplicon size 1177 bp
Locus specific primer CK04: 5'-TTCTCTTCTGTCAAATCAAACCCT-3'
2nd locus specific primer 17au: 5'-GATCCTTTGGATCTTAATTCGTCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11