Sequences from confirmation amplicon


Confirmation sequences for line 064A08 and hit At5g44160 are shown.
Line ID 064A08
Locus At5g44160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:17774663
Primers used for PCR 01kn - 8474, show primer details
   
Sequence Name 72-K113669-0022-064-A08C-01kn
GenBank Accession LN512760 [GenBank]
Sequencing primer gene specific primer, 01kn: 5'-TAGTGTCATGATTGAGGCAAAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K113669-0022-064-A08C-01kn
TAAGCTAGATATAATAATATATACAAATAATGGAGTACTTCATGTGTGTATGGTTCAAAA
TGGTAACATTATTTATTACTTAATATGCGGAAAAAGTGTCAACACAAGATTGACACATCA
ATAGTTATGTTACGGAAATATGTACTTAGGTTTTTCTCTACTTTTACTGCTGCACATAAA
GAAAAGGCAGCTAAATGTTTTCAAGTATTTTCATCAGTTAAAACTTTGATATAAATGCAT
TGAGATAAATGTTGATTAAGACGTTAACTAATGGTGGTTTTGGTTGAAACAGGCGAGACA
GCTTTATGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCGCACGNTTATTAT
   
Sequence Name 41-K113666-0022-064-A08C-8409
GenBank Accession LN504151 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K113666-0022-064-A08C-8409
ATTTACAATTGAATATATCCATAAAGCTGTCTCGCCTGTTTCAACCAAAACCACCATTAG
TTAACGTCTTAATCAACATTTATCTCAATGCATTTATATCAAAGTTTTAACTGATGAAAA
TACTTGAAAACATTTAGCTGCCTTTTCTTTATGTGCAGCAGTAAAAGTAGAGAAAAACCT
AAGTACATATTTCCGTAACATAACTATTGATGTGTCAATCTTGTGTTGACACTTTTTCCG
CATATTAAGTAATAAATAATGTTACCATTTTGAACCATACACACATGAAGTACTCCATTA
TTTGTATATATTATTATATCTAGCTTATGTTATATGTTCATCATGGTTATTATAAATTTA
TACCACTTTTTGCCTCATCANTGACACTAA

Last Updated on 06.12.2018 16:11