Genotyping and primer details


Genotyping and primer details for 064A08 - At5g44160
Line ID 064A08
Locus At5g44160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:17774663 of line 064A08 Chr5:17774663
   
Insertion allele amplicon 8474 - 01kn
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 01kn: 5'-TAGTGTCATGATTGAGGCAAAAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:17774295
   
wt amplicon (over InsPos) 10le - 01kn
wt amplicon start Chr5:17774295
wt amplicon end Chr5:17775267
wt amplicon size 973 bp
Locus specific primer 01kn: 5'-TAGTGTCATGATTGAGGCAAAAAG-3'
2nd locus specific primer 10le: 5'-AAACTTTTCGCCACTGTCATAACT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11