Sequences from confirmation amplicon


Confirmation sequences for line 066F10 and hit At3g20840 are shown.
Line ID 066F10
Locus At3g20840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7302572
Primers used for PCR 02jg - 8474, show primer details
   
Sequence Name 19-K112993-0022-066-F10C-02jg
GenBank Accession LN494835 [GenBank]
Sequencing primer gene specific primer, 02jg: 5'-TTTGTTCTTGATGCGTAAATCTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K112993-0022-066-F10C-02jg
TCGAATCGTATAAGATTAATTATATATAGTCTTTCATTAAAGGCAATTTAATTAAATCAA
ATTCTTGGTATAGGTGGATATGACAAACAATATAAAGCAGCAAGATCATATGATCTAGCT
GCACTTAAGTACTGGGGTCCTTCAACTACTACTAATTTCCCCGTATGTTAATTCATATTT
TTCTTGCAAATAATGTCGTGATACTAGTTTTGTATGCTTCAAATCTTTGATTCATGACTG
TATATCAGATTACAAACTACGAGAAAGAAGTAGAGGAAATGAAGCACATGACGAGACAAG
AGTTCGTGGCTGCCATTAGAAGGTTTTGTTTTGTGTTTTCCCATGAGGCATTTTCAAATA
AATTGAAACTTATCTAATCTAACAACTTATATATTCGTCAAATCACAGGAAAAGTAGTGG
ATTTTCGAGAGGCGCTTCGATGTATCGAGGAGTTACAAGGTTTGCATACGTAGATATTCC
CATGCTTAGTCTTTTAAGAAGCATCTTTACTTGTCGTTGAATTTTTTTTTGTTTTTCTTA
CTTATTATATGTATAGGCATCACCAACATGGAAGATGGCAAGCAAGGATCGGCCGAGTCG
CCGGAAACAAAGACCTCTACTTGGGAACTTTTAGTAAGTATCAGTTATTAGATCTTGACT
GCGTTTGGTACCATAAGAATTGATTCAAACGATTTAAATATTAGTACACAATTTTAAATC
AAAGAGGTTACTTTAGTAGAGAAAAATAGAAAGAACCTTACGCATATACATGCCAAAATC
AAAACATAACTTCAGAACCAGCCTGTATAAGTGTACGGTCCTGCAAACCATTATCTTACG
TTATCAATATGGTTTATCTTGTTCATGAAGCCATATTTACATGAAGCCAGGATATATTCA
ATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
   
Sequence Name 09-K112992-0022-066-F10C-8409
GenBank Accession LN490257 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K112992-0022-066-F10C-8409
ATTTCATTGAATTATCCTGGCTTCTGTAATATGGCTTCATGAACAAGATCATTATATTGA
TAACGTAAGATAATGGTTTGCAGGACCGTACACTTATACAGGCTGGTTCTGAAGTTATGT
TTTGATTTTGGCATGTATATGCGTAAGGTTCTTTCTATTTTTCTCTACTAAAGTAACCTC
TTTGATTTAAAATTGTGTACTAATATTTAAATCGTTTGAATCAATTCTTATGGTACCAAA
CGCAGTCAAGATCTAATAACTGATACTTACTAAAAGTTCCCAAGTAGAGGTCTTTGTTTC
CGGCGACTCGGCCGATCCTTGCTTGCCATCTTCCATGTTGGTGATGCCTATACATATAAT
AAGTAAGAAAAACAAAAAAAAATTCAACGACAAGTAAAGATGCTTCTTAAAAGACTAAGC
ATGGGAATATCTACGTATGCAAACCTTGTAACTCCTCGATACATCGAAGCGCCTCTCGAA
AATCCACTACTTTTCCTGTGATTTGACGAATATATAAGTTGTTAGATTAGATAAGTTTCA
ATTTATTTGAAAATGCCTCATGGGAAAACACAAAACAAAACCTTCTAATGGCAGCCACGA
ACTCTTGTCTCGTCATGTGCTTCATTTCCTCTACTTCTTTCTCGTAGTTTGTAATCTGAT
ATACAGTCATGAATCAAAGATTTGAAGCATACAAAACTAGTATCACGACATTATTTGCAA
GAAAAATATGAATTAACATACGGGGAAATTAGTAGTAGTTGAAGGACCCCAGTACTTAAG
TGCAGCTAGATCATATGATCTTGCTGCTTTATCTTCTTTGTCATATCCACCTATACCAAG
AATTTGATTTAATTAAGTTTCCTTTAATGAAAGACTATATATAATTAATCTTATACGATT
CGATGCACTCACCCAAGTAAACTAGAGATTATTATTGAGTCCCCAAGATTTACGCATCAA
AG

Last Updated on 06.12.2018 16:11