Genotyping and primer details


Genotyping and primer details for 066F10 - At3g20840
Line ID 066F10
Locus At3g20840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:7302572 of line 066F10 Chr3:7302572
   
Insertion allele amplicon 8474 - 02jg
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 02jg: 5'-TTTGTTCTTGATGCGTAAATCTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:7301659
   
wt amplicon (over InsPos) 12jz - 02jg
wt amplicon start Chr3:7301659
wt amplicon end Chr3:7302626
wt amplicon size 968 bp
Locus specific primer 02jg: 5'-TTTGTTCTTGATGCGTAAATCTTG-3'
2nd locus specific primer 12jz: 5'-AGCTATATCGTAAGCTTCTGCTGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11