Sequences from confirmation amplicon


Confirmation sequences for line 070F01 and hit At5g57290 are shown.
Line ID 070F01
Locus At5g57290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23207277
Primers used for PCR 07ew - 8474, show primer details
   
Sequence Name 49-K111119-0022-070-F01C-07ew
GenBank Accession LN506878 [GenBank]
Sequencing primer gene specific primer, 07ew: 5'-TCTTCGTGGTATCTCTCAGTCTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K111119-0022-070-F01C-07ew
AAAGTTGTGTGTTACTCGTAGATAGCTAGATCTTATAGATTTAGCTCGAATCTAGTGATG
CTTGTGATCACTTAATTTTGTTGCGATCATAATTTGTTTAGCTAACTAGTTGTGATAGAA
TCTTCAATTATGATTCCATTGGTGAAGAAGAATCTGAAAATTTGATTTGTATTTTAATGT
ATATTGTTTTTTAGCTACATTGTATTGTGTGTGTCATTTTGCTCTAATCTCATTTTCAAT
TTGGTGAAGCATACATGATACTTGTTTCATTGTTGAATTGTATGATGAGTTGTTGAAATG
AATGATACGAAGCTTAATTACCAATTTATATATTCAATATCCTGGCCTACAATTGTAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATGTAGATGTC
   
Sequence Name 33-K111118-0022-070-F01C-8409
GenBank Accession LN501041 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K111118-0022-070-F01C-8409
AGGCCAGGATATTGAATATATAAATTGGTAATTAAGCTTCGTATCATTCATTTCAACAAC
TCATCATACAATTCAACAATGAAACAAGTATCATGTATGCTTCACCAAATTGAAAATGAG
ATTAGAGCAAAATGACACACACAATACAATGTAGCTAAAAAACAATATACATTAAAATAC
AAATCAAATTTTCAGATTCTTCTTCACCAATGGAATCATAATTGAAGATTCTATCACAAC
TAGTTAGCTAAACAAATTATGATCGCAACAAAATTAAGTGATCACAAGCATCACTAGATT
CGAGCTAAATCTATAAGATCTAGCTATCTACGAGTAAACACACAACTTTATCACGATCCA
GAAATGAAAGAGATGAGACTGAGAGAACCAACGAAGA

Last Updated on 06.12.2018 16:11