Genotyping and primer details

Genotyping and primer details for 070F01 - At5g57290
Line ID 070F01
Locus At5g57290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:23207277 of line 070F01 Chr5:23207277
Insertion allele amplicon 8474 - 07ew
Locus specific primer 07ew: 5'-TCTTCGTGGTATCTCTCAGTCTCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:23207645
wt amplicon (over InsPos) 04fw - 07ew
wt amplicon start Chr5:23206925
wt amplicon end Chr5:23207645
wt amplicon size 721 bp
Locus specific primer 07ew: 5'-TCTTCGTGGTATCTCTCAGTCTCA-3'
2nd locus specific primer 04fw: 5'-AAAGCTGAAACCAGAAACAAACAT-3'
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11