Sequences from confirmation amplicon


Confirmation sequences for line 078B06 and hit At3g17910 are shown.
Line ID 078B06
Locus At3g17910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6134113
Primers used for PCR CD98 - 8474, show primer details
   
Sequence Name 15-K106018-0022-078-B06C-CD98
GenBank Accession LN492977 [GenBank]
Sequencing primer gene specific primer, CD98: 5'-GCATTAGAGGTGTGATGACAAAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K106018-0022-078-B06C-CD98
ACCGTGGACCCAATAGATAGACCTTTGCTCGTCAAAGACACCCTTGCAACTAACCCGTCT
AAATTCTAAAGCATTGAGATTCTTATCAAGAGGATGGTCTATATTTAGCTTTATTGGTTC
CATGTTCAACCGTTGCTGTTGATACTCCAGTGTTTTGAACTGCGGTAACAAGTAATGAAT
AAAGCTTTAGGGGTCTGCTCAGTGAAAAGCAACCAATTAATATTAAATGTCTCAGCTTCA
GCAACCATTTGGAAGAGAAAAAGATAGGAAATCAGGTAATGGAATAAGAGAAATCTCAAC
CAAGACATGGGCATGGTAGCCAGTACTCAGCATGCATTTACAATCAATAAGAGTGTCGCC
AGATCACTTATATGAAAGAATTACGAACATTTAGATTCTCACTGGAATCTGACATTTATT
CAACTTAATTAGTCTACATGACGATGCAGAGAACTGCATTGCGTCAGCATAGATCAGTAT
GTGCGGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCGCACGNTTTATT
   
Sequence Name 15-K106019-0022-078-B06C-8409
GenBank Accession LN492978 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K106019-0022-078-B06C-8409
GCCATTTACATTGAATTATCCCGCACATACTGATCTATGCTGACGCACTGTAGTTCTCTG
CATCGTCATGTAGACTAATTAAGTTGAATAAATGTCAGATTCCAGTGAGAATCTAAATGT
TCGTAATTCTTTCATATAAGTGATCTGGCGACACTCTTATTGATTGTAAATGCATGCTGA
GTACTGGCTACCATGCCCATGTCTTGGTTGAGATTTCTCTTATTCCATTACCTGATTTCC
TATCTTTTTCTCTTCCAAATGGTTGCTGAAGCTGAGACATTTAATATTAATTGGTTGCTT
TTCACTGAGCAGACCCCTAAAGCTTTATTCATTACTTGTTACCGCAGTTCAAAACACTGG
AGTATCAACAGCAACGGTTGAACATGGAACCAATAAAGCTAAATATAGACCATCCTCTTG
ATAAGAATCTCAATGCTTTAGAATTTAGACGGGTTAGTTGCAAGGGTGTCTTTGACGAGC
AAAGGTCTATCTATTTGGGTCCACGGTCTAGGTCCATATCGGGAATTACTGAAAATGGAT
TCTTTGTCATCACCCNTCTAATG
Primers used for PCR CD99 - 8474, show primer details
   
Sequence Name 36-K119006-0022-078-B06C-CD99
GenBank Accession LN502421 [GenBank]
Sequencing primer gene specific primer, CD99: 5'-CATCAATTTCTGAAAAGCTAAGCC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K119006-0022-078-B06C-CD99
TCACATTGTTGTTGTTCTTGCCTGGAGCGATTACCTTTGGTCTCGGGTCATGGCAGATCG
TCAGGAGAGAGGAAAAGGTCAGTACTTCGAAATCAATGGAAGTCCTTTCATCGTCTCAAG
CTTGGTTATAGCCTGTCTTAGACTTTTAATTGAAATTTATATTCAAAATGTGCTTCACAC
ATATTCTAGTTGTTTTTGTGATCCGTAAAGGAGTTAGAGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGATATTAT
   
Sequence Name 06-K119005-0022-078-B06C-8409
GenBank Accession LN489028 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K119005-0022-078-B06C-8409
CATTTCTCTAACTCCTTTACGGATCACAAAAACAACTAGAATATGTGTGAAGCACATTTT
GAATATAAATTTCAATTAAAAGTCTAAGACAGGCTATAACCAAGCTTGAGACGATGAAAG
GACTTCCATTGATTTCGAAGTACTGACCTTTTCCTCTCTCCTGACGATCTGCCATGACCC
GAGACCAAAGGTAATCGCTCCAGGCAAGAACAACAACAATTGTGACCACTTAGACCCTCG
TTTGTTCTCTGAAACAAAAAGGGCTTAGCTTTTCAGAAATTGATG
Primers used for PCR 21xf - 8474, show primer details
   
Sequence Name 37-K119006-0022-078-B06C-21xf
GenBank Accession LN502799 [GenBank]
Sequencing primer gene specific primer, 21xf: 5'-CTTCTTTCTTTCCGTGAAAACATC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K119006-0022-078-B06C-21xf
ATCTCGAGCTTTATATGTTTTGGAGTGAATCATAATCATAAAATGTTAGATGCTCGTGTT
TAATGACTGAAACACCAATGAACTTGTGTGTGTGACAGGTCGTAGATACATTCCAGAGCA
GTCACTGAAGATAAACGCAACTTCAAGCATTTCCTCTGGGATTTTCTAGTCTTTTACCCA
ATGCCACTTTTGCCTTTTTCTTTAGAGCCAAGAAGAGTCATCAAACACAGAGCAGTTCTA
TCTTCAGCATCTCCATAGAGACTCATCCAAAGATTGTGTGTTGAAAAATCGATTTTGGTT
TCCATGTTTCGAAGGTTTGAAGAAAAGTTGTTGTAATACCCCACTTGTGCTGAACCATGT
GTTTGCGTTCTTTAGCTTGCTTTTTGTAATTGATTGATTCTGAAATTCGCATAGAGACAG
AAGACGCAACCATTGTTATCGAATTTACATTTGTTATTTGCTTATAAAATCAGATAATTA
ACCAAAACTTTTCTTCAACAAATCCAAGTATGGCTAACAAATGAAGGACAAAAAGTTAGG
TGCACCGGAGCTTAATGGTGCACCCGAATGCAGGGGATAACAGCTCTACAAATCTGAAGC
CATTTTCACGGTGACCCCAAAGTCGTCGTGGAGGCAAAAACCCTTTACCTCCGGGATCAA
AATCTTGGGGAGAGACGAAGATGGCGACCTCGCTTTCCAAAATCCTAACGAGATCGAACA
CTAAGAGATACTGGTGCTCTACTACAACAAGTATCTCTGCCTCTCCTTCTTTACCGAAAC
AATTCTGGTCAAGACACTTCTCTGCCGTCGCTGACTCTTCCTCCTCTAGCTCCGCCGCTC
TTGGAAGCAATCGAAGTCTTCTGCTCCACCTCAAGGTATGTTGCTCGTGCTATG
   
Sequence Name 07-K119005-0022-078-B06C-oriL
GenBank Accession LN489493 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K119005-0022-078-B06C-oriL
TCTCTACTCCTTTACGGATCACAAAAACAACTAGAATATGTGTGAAGCACATTTTGAATA
TAAATTTCAATTAAAAGTCTAAGACAGGCTATAACCAAGCTTGAGACGATGAAAGGACTT
CCATTGATTTCGAAGTACTGACCTTTTCCTCTCTCCTGACGATCTGCCATGACCCGAGAC
CAAAGGTAATCGCTCCAGGCAAGAACAACAACAATTGTGACCACTTAGACCCTCGTTTGT
TCTCTGAAACAAAAAGGGCTTAGCTTTTCAGAAATTGATGCTTACTATCCTCAGATTCCA
AGCTCAAGCATTGGAAGACATAGCACGAGCAACATACCTTGAGGTGGAGCAGAAGAACTC
GATTGGCTTCCAAGAGCGGCGGAGCTAGAGGAGGAAGAGTCAGCGACGGCAGAGAAGTGT
CTTGACCAGAATTGTTTCGGTAAAGAAGGAGAGGCAGAGATACTTGTTGTAGTAGAGCAC
CAGTATCTCTTAGTGTTCGATCTCGTTAGGATTTTGGAAAGCGAGGTCGCCATCTTCGTC
TCTCCCCAAGATTTTGATCCCGGAGGTAAAGGGTTTTTGCCTCCACGACGACTTTGGGGT
CACCGTGAAAATGGCTTCAGATTTGTAGAGCTGTTATCCCCTGCATTCGGGTGCACCATT
AAGCTCCGGTGCACCTAACTTTTTGTCCTTCATTTGTTAGCCATACTTGGATTTGTTGAA
GAAAAGTTTTGGTTAATTATCTGATTTTATAAGCAAATAACAAATGTAAATTCGATAACA
ATGGTTGCGTCTTCTGTCTCTATGCGAATTTCAGAATCAATCAATTACAAAAAGCAAGCT
AAAGAACG

Last Updated on 06.12.2018 16:11