Genotyping and primer details


Genotyping and primer details for 078B06 - At3g17910
Line ID 078B06
Locus At3g17910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:6134113 of line 078B06 Chr3:6134113
   
Insertion allele amplicon 8474 - 21xf
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 21xf: 5'-CTTCTTTCTTTCCGTGAAAACATC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:6132851
   
Insertion allele amplicon 8474 - CD98
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CD98: 5'-GCATTAGAGGTGTGATGACAAAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:6134696
   
Insertion allele amplicon 8474 - CD99
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CD99: 5'-CATCAATTTCTGAAAAGCTAAGCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:6133834
   
wt amplicon (over InsPos) CD98 - CD99
wt amplicon start Chr3:6133834
wt amplicon end Chr3:6134696
wt amplicon size 863 bp
Locus specific primer CD99: 5'-CATCAATTTCTGAAAAGCTAAGCC-3'
2nd locus specific primer CD98: 5'-GCATTAGAGGTGTGATGACAAAGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11