Sequences from confirmation amplicon


Confirmation sequences for line 079C01 and hit At2g44190 are shown.
Line ID 079C01
Locus At2g44190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18273597
Primers used for PCR 06vf - 8474, show primer details
   
Sequence Name 42-K117914-0022-079-C01C-06vf
GenBank Accession LN504624 [GenBank]
Sequencing primer gene specific primer, 06vf: 5'-GAAGCAGAAGAAAGCTTTAGGACA-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K117914-0022-079-C01C-06vf
CGTTACTTGCAATGGCGATTTGCAAATGCAAATGCTGAGGTCAAAACACAATCTCAGAAA
GCACAAGCTGAGGTCAGTTTCTATAGCTCTGAGAATCAGAAATTTGATAGCTTTTACATA
TAGATCAATGTTTGACACATAACTTCAGTATTAGTGATGAATGTTGTTTAGGTTAGATTC
TGTTTGATGATGGTGATCTTATGGATGCTGTAAATTGTTTTTCTTGCAGAGAATGTTTTA
TTCACTTGGTTTGAAAATGTCGGAGTTGTCTGATTCTGTACAGAGGAAACGCATAGAACT
CCAACATTTACAGAGAGTTAAAGCTGTTACGGAGATTGTAGAGTCACAAGTAAGTAAAGA
TCTTAAATATCGTAGATTTCACACTATTTTTACATTTATGAACGTTTAGAGCAATTGTAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 02-K117913-0022-079-C01C-8409
GenBank Accession LN487221 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K117913-0022-079-C01C-8409
ATTGCTCTAAACGTTCATAAATGTAAAAATAGTGTGAAATCTACGATATTTAAGATCTTT
ACTTACTTGTGACTCTACAATCTCCGTAACAGCTTTAACTCTCTGTAAATGTTGGAGTTC
TATGCGTTTCCTCTGTACAGAATCAGACAACTCCGACATTTTCAAACCAAGTGAATAAAA
CATTCTCTGCAAGAAAAACAATTTACAGCATCCATAAGATCACCATCATCAAACAGAATC
TAACCTAAACAACATTCATCACTAATACTGAAGTTATGTGTCAAACATTGATCTATATGT
AAAAGCTATCAAATTTCTGATTCTCAGAGCTATAGAAACTGACCTCAGCTTGTGCTTTCT
GAGATTGTGTTTTGACCTCAGCATTTGCATTTGCAAATCGCCATTGCAAGTAACGGTTAT
GGAGCAGTTTCAGAGAATGCACATCCGCTTGTTGTCCTAAAGCTTTCTTCAGCAAAAAAA
AA

Last Updated on 06.12.2018 16:11