Genotyping and primer details


Genotyping and primer details for 079C01 - At2g44190
Line ID 079C01
Locus At2g44190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:18273597 of line 079C01 Chr2:18273597
   
Insertion allele amplicon 8474 - 06vf
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 06vf: 5'-GAAGCAGAAGAAAGCTTTAGGACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:18273122
   
wt amplicon (over InsPos) 34vu - 06vf
wt amplicon start Chr2:18273122
wt amplicon end Chr2:18274113
wt amplicon size 992 bp
Locus specific primer 06vf: 5'-GAAGCAGAAGAAAGCTTTAGGACA-3'
2nd locus specific primer 34vu: 5'-GCTTTCTCTATGCCGCTTACTCTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11